Riboflavin did not provide anti-inflammatory or antioxidant effects in an experimental model of sepsis
| Autor: | A.H.F. Vale, D.C. Nascimento, A.R. Pineros, R.G. Ferreira, J.D. Santos, D.C. Aragon, F.Q. Cunha, F.S. Ramalho, J.C. Alves-Filho, A.P.C.P. Carlotti |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Physiology
Multiple Organ Failure Riboflavin Organ dysfunction Immunology Anti-Inflammatory Agents Biophysics Ocean Engineering Biochemistry Antioxidants Mice Sepsis Animals Urea General Pharmacology Toxicology and Pharmaceutics Inflammation Tumor Necrosis Factor-alpha General Neuroscience Inflammatory response Bayes Theorem Cell Biology General Medicine Models Theoretical Anti-Bacterial Agents Mice Inbred C57BL Oxidative stress Creatinine Chemokines |
| Zdroj: | Brazilian Journal of Medical and Biological Research v.55 2022 Brazilian Journal of Medical and Biological Research Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC Brazilian Journal of Medical and Biological Research, Volume: 55, Article number: e12107, Published: 27 MAY 2022 |
| Popis: | We aimed to evaluate whether the administration of riboflavin to septic animals reduces inflammation, oxidative stress, organ dysfunction, and mortality. C57BL/6 mice, 6-8 weeks old, were allocated to the study group (polymicrobial sepsis induced by cecal ligation and puncture (CLP) + antibiotic + iv riboflavin), control (CLP + antibiotic + iv saline), or naïve (non-operated controls). Serum concentrations of alanine aminotransferase (ALT), creatine kinase-MB (CK-MB), urea, and creatinine, and markers of inflammation [interleukin (IL)-6, tumor necrosis factor (TNF)-α, keratinocyte-derived chemokine (KC), and macrophage inflammatory protein (MIP)-2)], and oxidative stress (malondialdehyde (MDA) were measured 12 h after the experiment. Animal survival rates were calculated after 7 days. Means between groups were compared using linear regression models adjusted under the Bayesian approach. No significant difference was observed between control and study groups in serum concentrations of IL-6 (95% credible interval) (-0.35 to 0.44), TNF-α (-15.7 to 99.1), KC (-0.13 to 0.05), MIP-2 (-0.84 to 0.06), MDA (-1.25 to 2.53), or ALT (-6.6 to 11.5). Serum concentrations of CK-MB (-145.1 to -30.1), urea (-114.7 to -15.1), and creatinine (-1.14 to -0.01) were higher in the study group. Survival was similar in both groups (P=0.8). Therefore, the use of riboflavin in mice undergoing sepsis induced by CLP did not reduce inflammation, oxidative stress, organ dysfunction, or mortality compared with placebo. |
| Databáze: | OpenAIRE |
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