Imaging atrial arrhythmic intracellular calcium in intact heart
| Autor: | Andrew R. Marks, Gaetano Santulli, Xiaoxiao Guo, Bi Xing Chen, Wenjun Xie, Melanie Gao |
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| Přispěvatelé: | Xie, Wenjun, Santulli, Gaetano, Guo, Xiaoxiao, Gao, Melanie, Chen, Bi Xing, Marks, Andrew R. |
| Rok vydání: | 2013 |
| Předmět: |
Intracellular Ca(2+) imaging
medicine.medical_specialty Intracellular Space chemistry.chemical_element In Vitro Techniques Calcium Ryanodine receptor 2 Article Calcium in biology Molecular Imprinting Electrocardiography Mice Internal medicine medicine Animals Myocytes Cardiac Calcium Signaling Heart Atria cardiovascular diseases Molecular Biology Calcium signaling Mice Knockout medicine.diagnostic_test Animal In Vitro Technique Ryanodine receptor Endoplasmic reticulum Arrhythmias Cardiac Ryanodine Receptor Calcium Release Channel Atrial arrhythmia Disease Models Animal Endocrinology chemistry cardiovascular system Cardiology and Cardiovascular Medicine Ex vivo model Intracellular |
| Zdroj: | Journal of Molecular and Cellular Cardiology. 64:120-123 |
| ISSN: | 0022-2828 |
| DOI: | 10.1016/j.yjmcc.2013.09.003 |
| Popis: | Abnormalities in intracellular Ca(2+) signaling have been proposed to play an essential role in the pathophysiology of atrial arrhythmias. However, a direct observation of intracellular Ca(2+) in atrial myocytes during atrial arrhythmias is lacking. Here, we have developed an ex vivo model of simultaneous Ca(2+) imaging and electrocardiographic recording in cardiac atria. Using this system we were able to record atrial arrhythmic intracellular Ca(2+) activities. Our results indicate that atrial arrhythmias can be tightly linked to intracellular Ca(2+) waves and Ca(2+) alternans. Moreover, we applied this strategy to analyze Ca(2+) signals in the hearts of WT and knock-in mice harboring a 'leaky' type 2 ryanodine receptor (RyR2-R2474S). We showed that sarcoplasmic reticulum (SR) Ca(2+) leak increases the susceptibility to Ca(2+) alternans and Ca(2+) waves increasing the incidence of atrial arrhythmias. Reduction of SR Ca(2+) leak via RyR2 by acute treatment with S107 reduced both Ca(2+) alternans and Ca(2+) waves, and prevented atrial arrhythmias. |
| Databáze: | OpenAIRE |
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