Identification of sialic acid linkage isomers in glycans using coupled InfraRed Multiple Photon Dissociation (IRMPD) spectroscopy and mass spectrometry
| Autor: | Agathe Depraz Depland, Baptiste Schindler, Isabelle Compagnon, Gina Renois-Predelus |
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| Přispěvatelé: | Institut Lumière Matière [Villeurbanne] (ILM), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Institut Universitaire de France (IUF), Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.) |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Glycan IRMPD spectroscopy Stereochemistry Infrared spectroscopy Protonation Mass spectrometry 01 natural sciences [SPI]Engineering Sciences [physics] 03 medical and health sciences chemistry.chemical_compound Ion spectroscopy [CHIM]Chemical Sciences Infrared multiphoton dissociation Physical and Theoretical Chemistry Linkage isomerism Spectroscopy Glycomics Instrumentation [PHYS]Physics [physics] biology N-glycan epitopes 010401 analytical chemistry Human milk oligosaccharides Condensed Matter Physics Sialic acid 0104 chemical sciences carbohydrates (lipids) 030104 developmental biology chemistry biology.protein |
| Zdroj: | International Journal of Mass Spectrometry International Journal of Mass Spectrometry, Elsevier, 2018, 434, pp.65-69. ⟨10.1016/j.ijms.2018.09.005⟩ |
| ISSN: | 1387-3806 |
| DOI: | 10.1016/j.ijms.2018.09.005 |
| Popis: | International audience; The gas phase IR spectra of α2,3 and α2,6 linkage isomers of sialic acid-containing human milk oligosaccharides and N-glycans epitopes are measured in the 3 µm spectral range using IRMPD spectroscopy in protonated and Na+ charge states. Each linkage isomer has a unique IRMPD fingerprint, providing a convenient diagnostic of the sialic acid position. Finally, a generic trend is observed: 3-linked sialic acid results in a significant broadening of the spectroscopic signature regardless of the glycan core and the charge state which suggests a drastic influence of the sialic acid position on the glycan conformation. |
| Databáze: | OpenAIRE |
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