Response of the chick ciliary ganglion-iris neuromuscular preparation to botulinum neurotoxin
| Autor: | Rich Lomneth, Bibhuti R. DasGupta, Janusz B. Suszkiw |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Botulinum Toxins
Neuromuscular Junction Iris Biology In Vitro Techniques medicine.disease_cause Synaptic Transmission Neuromuscular junction medicine Neurotoxin Animals General Neuroscience Neurotoxicity Ciliary ganglion Ganglia Parasympathetic Anatomy Trypsin medicine.disease Molecular biology medicine.anatomical_structure Ciliary muscle nervous system Clostridium botulinum medicine.symptom Chickens Muscle contraction medicine.drug |
| Zdroj: | Neuroscience letters. 113(2) |
| ISSN: | 0304-3940 |
| Popis: | Response of the chick ciliary ganglion-iris muscle neuromuscular junction (NMJ) preparation to the botulinum neurotoxin (NT) was investigated. The 150 kDa serotypes A and E NTs inhibited muscle contraction in a dose dependent fashion. Neurotoxicity of type E NT increased 20–40 fold after mild digestion with trypsin. The 50 kDA light and 100 kDa heavy chains of type A NT, following separation, applied individually, did not paralyze the tissues. Preincubation of the NMJ preparations with the isolated type A heavy chain delayed (antagonized) the paralytic action of the 150 kDa dichain type A NT. Sequential administration of type A heavy chain, followed by type A light chain mimicked the action of the parent NT. The chick ciliary preparation therefore is a useful NMJ preparation to study neurotoxicity of botulinum neurotoxins. |
| Databáze: | OpenAIRE |
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