Chronic ingestion of Primex-Z, compared with other common fat sources, drives worse liver injury and enhanced susceptibility to bacterial infections
| Autor: | Hortência Maciel de Castro-Oliveira, Rafael M. Rezende, Raquel Carvalho-Gontijo, Kassiana Mafra Bicalho, Mateus Eustáquio Lopes, Matheus Silvério Mattos, Camila Dutra Moreira de Miranda, Gabriel Alvim Machado Mendes, Ariane Barros Diniz, Alesandra Corte Reis Melão, Brenda Naemi Nakagaki, Gustavo B. Menezes, Karen Costa, Matthew Ricci, Maria Alice Freitas-Lopes, Sridhar Radhakrishnnan, Maísa Mota Antunes |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Trans fat Calorie Endocrinology Diabetes and Metabolism 030209 endocrinology & metabolism Biology Diet High-Fat 03 medical and health sciences Eating Mice 0302 clinical medicine Non-alcoholic Fatty Liver Disease Internal medicine medicine Ingestion Animals Liver injury 030109 nutrition & dietetics Nutrition and Dietetics Fatty liver Bacterial Infections medicine.disease Mice Inbred C57BL Endocrinology Liver medicine.symptom Steatosis Weight gain Corn oil |
| Zdroj: | Nutrition (Burbank, Los Angeles County, Calif.). 81 |
| ISSN: | 1873-1244 |
| Popis: | Objectives To investigate putative different outcomes on non-alcoholic fatty liver disease development in mice using fat options regularly used in human nutrition. Methods Male C57BL/6 mice were fed control diet, and four different high fat diets (HFD - 40% calories from fat, Research Diets, USA) for 16 and 30 weeks. High fat diets had different common fat sources, including trans fat, non-trans fat palm oil (Primex-Z®), palm oil alone and corn oil alone. Mice were euthanized and samples were collected for analysis. Results Using an unprecedented combination of in vivo imaging with immunometabolic phenotyping, we revealed that HFD induced a major increase in hepatic lipid droplet deposition in comparison to controls, being significantly higher in Primex-Z® fed mice. All HFDs had similar or less weight gain compared with control mice; however, Primex-Z® ingestion led to a higher increasing in adiposity index (∼90% increase) in comparison to other fat sources. Gene expression of isolated liver immune cells revealed large changes expression of several inflammatory pathways, which were also more elevated in Primex-Z fed mice, including Tnf (∼20-fold), Il1b (∼60-fold) and Tgfb (2.5-fold). Immunophenotyping and in vivo analysis showed that the frequency of hepatic immune cells was also disturbed during different HFD contents, rendering not only Kupffer cell depletion, but also reduced bacterial arresting ability. Conclusion Different fat dietary sources imprint different immune and metabolic impacts in the liver during high fat diet consumption. Our data highlight that Primex-Z® – a novel non-trans fat – is not only able to damage hepatocytes, but also impair liver ability to clear blood-borne infections. |
| Databáze: | OpenAIRE |
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