Flavonoid Mixture Inhibits Mycobacterium tuberculosis Survival and Infectivity
| Autor: | Garrett Teskey, David C. Nieman, Myra Gutierrez, Marcel Fraix, Airani Sathananthan, Ruoqiong Cao, Vishwanath Venketaraman, Hicret Islamoglu, Shalok Munjal, Oscar Salaiz |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Male
THP-1 Cells medicine.medical_treatment Pharmaceutical Science immunomodulation Analytical Chemistry chemistry.chemical_compound 0302 clinical medicine Drug Discovery glutathione Infectivity 0303 health sciences biology Middle Aged Cytokine TB Chemistry (miscellaneous) Molecular Medicine Cytokines Female Intracellular Adult Programmed cell death Tuberculosis T2DM Article Microbiology Mycobacterium tuberculosis lcsh:QD241-441 03 medical and health sciences lcsh:Organic chemistry medicine Humans Physical and Theoretical Chemistry polyphenols 030304 developmental biology Microbial Viability Mtb Organic Chemistry Glutathione medicine.disease biology.organism_classification In vitro chemistry Diabetes Mellitus Type 2 flavonoids 030215 immunology |
| Zdroj: | Molecules, Vol 24, Iss 5, p 851 (2019) Molecules Volume 24 Issue 5 |
| ISSN: | 1420-3049 |
| Popis: | Background: Flavonoids have been shown to exert anti-pathogenic potential, but few studies have investigated their effects on Mycobacterium tuberculosis (Mtb) infectivity. We hypothesized that a flavonoid mixture would have a favorable influence on cell death and the resolution of Mtb infection in THP-1 macrophages and in granulomas derived from both healthy participants and those with type 2 diabetes mellitus (T2DM). METHODS: THP-1 macrophages, and in vitro granulomas from healthy participants (N = 8) and individuals with T2DM (N = 5) were infected with Mtb. A mixed flavonoid supplement (MFS) at a concentration of 0.69 mg per ml was added as treatment to Mtb infected THP-1 macrophages and granulomas for 8 to 15 days. RESULTS: MFS treatment significantly reduced the intracellular Mtb survival, increased cell density, aggregation, and granuloma formation, and increased glutathione (GSH) levels. IL-12 and IFN-&gamma levels tended to be higher and IL-10 lower when Mtb infected THP-1 macrophages and granulomas obtained from healthy subjects were treated with MFS compared to control. CONCLUSIONS: MFS treatment exerted a strong influence against Mtb infectivity in THP-1 macrophages and in granulomas including antimycobacterial effects, GSH enrichment, cytokine regulation, and augmented granuloma formation. Our data support the strategy of increased flavonoid intake for managing tuberculosis. |
| Databáze: | OpenAIRE |
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