The further characterization of the peptide specifically binding to gastric cancer
| Autor: | Wei Duan, Juanjuan Han, Yingzhuo Huang, Feng-Huei Lin, Qinqin Xue, Yan Duan, Yingchun Hou, Xiaojie Gao, Qian Wang, Guochao Nie |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Adult Male Fluorescent Antibody Technique Peptide Gastric carcinoma Biology Bioinformatics Fluorescence Flow cytometry 03 medical and health sciences 0302 clinical medicine Stomach Neoplasms Cell Line Tumor medicine Fluorescence microscope Humans Molecular Biology Aged chemistry.chemical_classification medicine.diagnostic_test Cell Death Targeting therapy Cancer Cell Biology Middle Aged medicine.disease Flow Cytometry Small molecule 030104 developmental biology HEK293 Cells chemistry Tissue Array Analysis 030220 oncology & carcinogenesis Cancer cell Cancer research Female Peptides Protein Binding |
| Zdroj: | Molecular and cellular probes. 30(3) |
| ISSN: | 1096-1194 |
| Popis: | Targeting peptide has been considered to be useful as a small molecule probe leading to multifunctional properties for both imaging detection and targeting therapy. Thus, the identification of novel targets is urgently needed to develop innovative agents to effectively control gastric cancer metastasis and progression. Previously, we reported a novel 12-mer peptide, GP-5 (IHKDKNAPSLVP), binding to gastric carcinoma (GC) cells specifically and sensitively, and it was screened by using a phage displayed peptide library and primarily analyzed. In this study, it was further identified via fluorescence microscopy, flow cytometry, tissue chip and other methods. Our results indicated that the peptide GP-5 presents a particularly high affinity and specificity to GC cells and tissues, whereas only background detection occurred with other control cancer cells, cancer tissues or normal tissues. Taken together, all results support that the peptide GP-5 is a potential candidate to be developed as a useful molecule fragment for the imaging detection and targeting therapy of GC. |
| Databáze: | OpenAIRE |
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