Induction of Potent Th1‐Type Immune Responses from a Novel DNA Vaccine for West Nile Virus New York Isolate (WNV‐NY1999)
| Autor: | J. Joseph Kim, Mathura P. Ramanathan, Joo-Sung Yang, Daniel Hwang, David B. Weiner, Andrew Y. Choo, Henry C. Maguire, Sagar B. Kudchodkar, Kesen Dang |
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| Rok vydání: | 2001 |
| Předmět: |
animal diseases
viruses Dengue virus Biology medicine.disease_cause Virus DNA vaccination Mice Flaviviridae Capsid Kunjin virus Veterinary virology Vaccines DNA medicine Animals Immunology and Allergy Antigens Viral Mice Inbred BALB C Muscles virus diseases Viral Vaccines Th1 Cells Japanese encephalitis medicine.disease biology.organism_classification Virology Disease Models Animal Flavivirus Infectious Diseases Cytokines Female West Nile virus West Nile Fever T-Lymphocytes Cytotoxic |
| Zdroj: | The Journal of Infectious Diseases. 184:809-816 |
| ISSN: | 1537-6613 0022-1899 |
| DOI: | 10.1086/323395 |
| Popis: | West Nile virus (WNV) is a vectorborne pathogen that induces brain inflammation and death. Recently, confirmed cases of infection and deaths have occurred in the United States Mid-Atlantic region. In this study, a DNA vaccine encoding the WNV capsid protein was constructed, and the in vivo immune responses generated were investigated in DNA vaccine‐immunized mice. Antigen-specific humoral and cellular immune responses were observed, including a potent induction of antigen-specific Th1 and cytotoxic T lymphocyte responses. Strong induction of Th1-type immune responses included high levels of antigen-specific elaboration of the Th1-type cytokines interferon-g and interleukin-2 and b-chemokines RANTES (regulated upon activation, normal T cell-expressed and secreted) and macrophage inflammatory protein‐1b. Dramatic infiltration of CD4 and CD8 T cells and macrophages also was observed at the muscle injection site. These results support the potential utility of this method as a tool for developing immunization strategies for WNV and other emerging pathogens. West Nile virus (WNV), a single-stranded RNA virus, is a member of the Flaviviridae family, a group that includes the St. Louis encephalitis virus, Kunjin virus, yellow fever virus, Dengue virus, and Japanese encephalitis virus. WNV usually is transmitted by Culex species mosquitoes, and wild birds are its normal hosts. WNV was first isolated from the blood of a febrile Ugandan woman in 1937, and outbreaks of WNV have been reported throughout Africa, the Middle East, and parts of Europe and western Asia [1]. However, WNV was not seen in North America until late summer 1999, when a WNV encephalitis outbreak occurred in New York City and vicinity and resulted in the infection of 61 people and 7 deaths (as of 21 |
| Databáze: | OpenAIRE |
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