PLGA nanoparticles containing α-fetoprotein siRNA induce apoptosis and enhance the cytotoxic effects of doxorubicin in human liver cancer cell line
| Autor: | Kytai T. Nguyen, Chayapol Somboonyosdech, Theeraphong Pho-iam, Vorapan Sirivatanauksorn, Sirinapa Sripinitchai, Primana Punnakitikashem, Chatchawan Srisawat, Yongyut Sirivatanauksorn, Chamaiphorn Wongwan, Patarabutr Masaratana |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Programmed cell death Small interfering RNA Carcinoma Hepatocellular Biophysics Apoptosis Biochemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Polylactic Acid-Polyglycolic Acid Copolymer medicine Humans Cytotoxic T cell Doxorubicin RNA Small Interfering Cytotoxicity neoplasms Molecular Biology Liver Neoplasms Hep G2 Cells Cell Biology digestive system diseases PLGA 030104 developmental biology chemistry Cell culture 030220 oncology & carcinogenesis Cancer research Nanoparticles alpha-Fetoproteins medicine.drug |
| Zdroj: | Biochemical and Biophysical Research Communications. 553:191-197 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2021.03.086 |
| Popis: | Hepatocellular carcinoma (HCC) is one of the most common cancers and is a leading cause of death. Delivery of therapeutic molecules, e.g., siRNA, to HCC cells could potentially be an alternative treatment for HCC. In this study, the siRNA targeting α-fetoprotein (AFP) mRNA was found to specifically induce apoptosis and significant cell death in HepG2 cells. It also enhanced the cytotoxic effects of doxorubicin by about two-fold, making it the candidate therapeutic molecule for HCC treatment. To deliver the siRNAs into HCC cells, the AFP siRNAs were loaded into the nanoparticles based on poly (lactic-co-glycolic) acid (PLGA). These nanoparticles induced apoptosis in HepG2 cells and synergistically increased the cytotoxicity of doxorubicin. In summary, the delivery of the AFP siRNA-loaded PLGA nanoparticles in combination with doxorubicin could be a very promising approach for the treatment of HCC. |
| Databáze: | OpenAIRE |
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