In Vivo Evaluation of a Bombesin Analogue Labeled with Ga-68 and Co-55/57

Autor: Poul Flemming Høilund-Carlsen, Christina Baun, Johan Hygum Dam, Helge Thisgaard, Birgitte Brinkmann Olsen
Rok vydání: 2015
Předmět:
Male
Bombesin/analogs & derivatives
Cancer Research
Peptides/blood
Peptide
Mice
SCID
030218 nuclear medicine & medical imaging
chemistry.chemical_compound
0302 clinical medicine
Radiopharmaceuticals/chemistry
Tissue Distribution
Gallium Radioisotopes/chemistry
Cobalt Radioisotopes
Chromatography
High Pressure Liquid
chemistry.chemical_classification
Prostate cancer
medicine.diagnostic_test
Protein Stability
Stomach
Gallium-68
Bombesin
medicine.anatomical_structure
Oncology
Positron emission tomography
030220 oncology & carcinogenesis
Pancreas
Preclinical imaging
Biodistribution
AMBA
Gallium Radioisotopes
Binding
Competitive
Inhibitory Concentration 50
03 medical and health sciences
In vivo
Cell Line
Tumor
medicine
Animals
Humans
Radiology
Nuclear Medicine and imaging
business.industry
Cobalt Radioisotopes/chemistry
Xenograft Model Antitumor Assays
PET
chemistry
Positron-Emission Tomography
Radiopharmaceuticals
Peptides
Tomography
X-Ray Computed
Nuclear medicine
business
Cobalt-55
Zdroj: Dam, J H, Olsen, B B, Baun, C, Høilund-Carlsen, P F & Thisgaard, H 2016, ' In Vivo Evaluation of a Bombesin Analogue Labeled with Ga-68 and Co-55/57 ', Molecular Imaging and Biology, vol. 18, no. 3, pp. 368-376 . https://doi.org/10.1007/s11307-015-0911-z
ISSN: 1860-2002
1536-1632
DOI: 10.1007/s11307-015-0911-z
Popis: Purpose: The purpose of this study was to apply an analogue of bombesin, NOTA-AMBA, labeled with Co-55 or Ga-68, for preclinical imaging of prostate cancer. Procedures: The peptide NOTA-AMBA was labeled with Ga-68 or Co-55 by microwave irradiation. Biodistribution in xenograft mice (PC3) was performed at 1, 4, and 24 h (only cobalt at 24 h) using a fixed amount of peptide. Four weeks post-inoculation, xenograft mice were positron emission tomography/X-ray computed tomography scanned after tail vein injection of [ 68Ga]NOTA-AMBA or [ 55Co]NOTA-AMBA. Results: Labeling with Ga-68 and Co-55/57 was achieved in yields greater than 90 %. A radiochemical purity (RCP) of 95 and 90 % were obtained for Ga-68 and Co-55, respectively. Both radiopeptides showed high uptake in the intestines, stomach, pancreas, and in the tumor ([ 68Ga]NOTA-AMBA, 10.3 %ID/g at 1 h to 6.4 %ID/g at 4 h; [ 57Co]NOTA-AMBA, 8.2 %ID/g at 1 h to 5.3%ID/g at 24 h). Normal tissue cleared over time improving tumor-to-background ratios. Conclusions: NOTA-AMBA was labeled in high yields and RCP with Ga-68 and Co-55/57. High tumor uptake in a subcutaneous mouse prostate cancer model was observed. At 24 h, [ 55/57Co]NOTA-AMBA showed better tumor-to-organ ratios than [ 68Ga]NOTA-AMBA at both 1 and 4 h post-injection. Hence, for imaging, [ 55Co]NOTA-AMBA was found to be superior compared to [ 68Ga]NOTA-AMBA.
Databáze: OpenAIRE
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