Magnetic Targeting of Growth Factors Using Iron Oxide Nanoparticles
| Autor: | Orit Shefi, Shlomo Margel, Menachem Motiei, Amos Sharoni, Michal Marcus, Ziv Shemesh, Ahmad Maswadeh, Idan Zak, Hadas Schori, Alexandra Smith |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
magnetic nanoparticles General Chemical Engineering 02 engineering and technology Article nerve growth factor lcsh:Chemistry 03 medical and health sciences chemistry.chemical_compound In vivo medicine General Materials Science neuronal regeneration Regeneration (biology) Sciatic nerve injury equipment and supplies 021001 nanoscience & nanotechnology medicine.disease sciatic nerve injury magnetic targeting 030104 developmental biology lcsh:QD1-999 chemistry Magnet Drug delivery Biophysics Magnetic nanoparticles 0210 nano-technology Drug carrier human activities Iron oxide nanoparticles |
| Zdroj: | Nanomaterials, Vol 8, Iss 9, p 707 (2018) Nanomaterials Volume 8 Issue 9 |
| ISSN: | 2079-4991 |
| DOI: | 10.3390/nano8090707 |
| Popis: | Growth factors play an important role in nerve regeneration and repair. An attractive drug delivery strategy, termed &ldquo magnetic targeting&rdquo aims to enhance therapeutic efficiency by directing magnetic drug carriers specifically to selected cell populations that are suitable for the nervous tissues. Here, we covalently conjugated nerve growth factor to iron oxide nanoparticles (NGF-MNPs) and used controlled magnetic fields to deliver the NGF&ndash MNP complexes to target sites. In order to actuate the magnetic fields a modular magnetic device was designed and fabricated. PC12 cells that were plated homogenously in culture were differentiated selectively only in targeted sites out of the entire dish, restricted to areas above the magnetic &ldquo hot spots&rdquo To examine the ability to guide the NGF-MNPs towards specific targets in vivo, we examined two model systems. First, we injected and directed magnetic carriers within the sciatic nerve. Second, we injected the MNPs intravenously and showed a significant accumulation of MNPs in mouse retina while using an external magnet that was placed next to one of the eyes. We propose a novel approach to deliver drugs selectively to injured sites, thus, to promote an effective repair with minimal systemic side effects, overcoming current challenges in regenerative therapeutics. |
| Databáze: | OpenAIRE |
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