Antibacterial activity of erythrocyte from grass carp (Ctenopharyngodon idella) is associated with phagocytosis and reactive oxygen species generation
| Autor: | Zaohe Wu, Lijuan Zhao, Yunjia Dai, Jungang Xie, Li Lin, Hanzuo Lin, Sarath Babu Vijayaraman, Zhendong Qin, Weiqiang Lin, Jun Li |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Staphylococcus aureus Proteases Carps Erythrocytes Phagocytosis Aquatic Science Methemoglobin 03 medical and health sciences Escherichia coli Extracellular Animals Environmental Chemistry chemistry.chemical_classification Reactive oxygen species biology 04 agricultural and veterinary sciences General Medicine biology.organism_classification Erythrophagocytosis Aeromonas hydrophila Anti-Bacterial Agents Grass carp 030104 developmental biology Biochemistry chemistry 040102 fisheries 0401 agriculture forestry and fisheries Hemoglobin Reactive Oxygen Species |
| Zdroj: | Fish & Shellfish Immunology. 92:331-340 |
| ISSN: | 1050-4648 |
| DOI: | 10.1016/j.fsi.2019.06.008 |
| Popis: | Red blood cells (RBCs) are widely accepted as their primary function in respiration. Recent studies in mammals have revealed a vital role in immune responses of RBCs; however, little is known about immune function of teleost erythrocytes. Here we demonstrated that RBCs from grass carp (Ctenopharyngodon idella) were capable of binding and aggregating the bacteria with apparent morphological alterations. The phagocytosis by teleost RBCs (erythrophagocytosis) was visualized by confocal, scanning and transmission electron microscopy. Hb-FeII of hemoglobin (Hb) could quickly be auto-oxidated to Hb-FeIII (methemoglobin/metHb) in the presence of oxygen (O2), and release superoxide radical (O2-.) which could be spontaneously dismutated into H2O2 that could further oxidize Hb-FeIII to transient HbFeIV-OH (ferryl-Hb). Furthermore, bacterial extracellular proteases and pathogen-associated molecular patterns (PAMPs) binding to Hb could synergistically activate pseudoperoxidase, subsequently facilitated the generation of reactive oxygen species (ROS) which were toxic to the bacteria. Our results indicated that erythrocyte pertains anti-bacterial activity using unique ROS generation pathway via oxidation of hemoglobin and associated with its phagocytosis. |
| Databáze: | OpenAIRE |
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