Tubeless biochip for tailoring cell co-cultures in closed microchambers
| Autor: | Fabio Beltram, Orazio Vittorio, Marco Cecchini, Sandro Meucci |
|---|---|
| Přispěvatelé: | Meucci, Sandro, Vittorio, Orazio, Beltram, Fabio, Cecchini, Marco |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Fluorescence-lifetime imaging microscopy
Materials science Microfluidics Nucleation Nanotechnology Nanoengineering Condensed Matter Physics Atomic and Molecular Physics and Optics Surfaces Coatings and Films Electronic Optical and Magnetic Materials Tubeless Microfluidic chip Cell filter Electrical and Electronic Engineering Biochip Migration Co-cultures Microchamber |
| Zdroj: | Microelectronic engineering 124 (2014): 8–12. doi:10.1016/j.mee.2014.04.015 info:cnr-pdr/source/autori:Meucci S.[ 1,2,3 ]; Vittorio O.[ 1,2,3 ]; Beltram F.[ 1,2,3 ]; Cecchini M. [1,2 ]/titolo:Tubeless biochip for tailoring cell co-cultures in closed microchambers/doi:10.1016%2Fj.mee.2014.04.015/rivista:Microelectronic engineering/anno:2014/pagina_da:8/pagina_a:12/intervallo_pagine:8–12/volume:124 |
| DOI: | 10.1016/j.mee.2014.04.015 |
| Popis: | Display Omitted We developed a tubeless microfluidic chip for cell co-culturing.On-chip cell filters were used to implement automated cell-loading.Topographically controlled co-cultures were realized by serial cell loadings.Cell migration within a closed microchamber was monitored for 72h. Lab-on-a-Chip devices exploit micro/nanoengineering techniques to develop miniaturized systems for handling and manipulating small volumes of fluids. Here, we demonstrate a microfluidic device for culturing cells in closed microchambers that is designed to be compatible with standard biological procedures. This approach facilitates the thermal and gaseous equilibration of all the components, preventing the nucleation of air bubbles. Serial asymmetrical loadings were performed to realize co-cultures with initial topographical organization. The migration of two different cell types was monitored for 72h by high-resolution fluorescence imaging. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|