Linezolid Diminishes Inflammatory Cytokine Production from Human Peripheral Blood Mononuclear Cells
| Autor: | Pilar Garcia-Roca, Araceli Santos-Segura, Javier Mancilla-Ramírez, Ernesto Calderón-Jaimes, Marisol Fernández-Avilés |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Lipopolysaccharides
medicine.drug_class medicine.medical_treatment Antibiotics Drug Evaluation Preclinical Erythromycin Peripheral blood mononuclear cell Monocytes chemistry.chemical_compound Acetamides medicine Humans Cells Cultured Oxazolidinones Inflammation Protein Synthesis Inhibitors Venipuncture Dose-Response Relationship Drug business.industry Linezolid General Medicine Antimicrobial Staining Cytokine chemistry Protein Biosynthesis Immunology Cytokines business medicine.drug |
| Zdroj: | Archives of Medical Research. 37:31-35 |
| ISSN: | 0188-4409 |
| DOI: | 10.1016/j.arcmed.2005.05.022 |
| Popis: | Background Active peptides produced by monocytes, in response to endotoxin, initiate and maintain the acute phase of inflammatory response. Some antibiotics have been reported to have immunomodulatory effects in addition to their antimicrobial activity. We examined the effect of linezolid on cytokine synthesis. Methods The modulatory effects of erythromycin and linezolid were evaluated in LPS-stimulated human peripheral blood mononuclear cells (PBMCs). Blood was obtained by venipuncture from healthy donor volunteers. PBMCs were separated by Ficoll-Paque. More than 90% of the cells were monocytes as determined by esterase staining. Cells were incubated in the presence of LPS, with or without various concentrations of erythromycin and linezolid. The concentration of each cytokine was determined by ELISA with commercially available reagents. Results The two drugs suppressed significantly the synthesis of the cytokines tested in a concentration-dependent manner. Conclusions These data indicate that antibacterial agents may modify acute phase inflammatory response through their effects on cytokines synthesis by monocytes. |
| Databáze: | OpenAIRE |
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