A double knockout mutant of acyl-glucose-dependent anthocyanin glucosyltransferase genes in Delphinium grandiflorum
| Autor: | Izumi Ishii, Yoshihiro Ozeki, Kimitoshi Sakaguchi, Kazuyoshi Fujita, Taira Miyahara |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine DNA Complementary Physiology Acylation Mutant Plant Science Flowers medicine.disease_cause Genes Plant 01 natural sciences law.invention Anthocyanins 03 medical and health sciences Gene Knockout Techniques Glucosyltransferases law Gene Expression Regulation Plant medicine Gene Escherichia coli Plant Proteins biology fungi Nucleic acid sequence Delphinium biology.organism_classification carbohydrates (lipids) 030104 developmental biology Glucose Biochemistry Mutation biology.protein Recombinant DNA Glucosyltransferase Agronomy and Crop Science Delphinium grandiflorum 010606 plant biology & botany |
| Zdroj: | Journal of plant physiology. 216 |
| ISSN: | 1618-1328 |
| Popis: | Blue coloration in delphinium flowers arises from 7-polyacylated anthocyanins which are modified alternately with acyl and glucosyl residues at the 7 position of the aglycone. Previously, we identified two independent genes for acyl-glucose-dependent anthocyanin 7-(6-(p-hydroxybenzoyl)-glucoside) glucosyltransferases (AA7BG-GT); recombinant proteins from the two cDNAs were produced in Escherichia coli and showed AA7BG-GT activity in vitro. Here, a double knockout mutant of both genes was found to lack modification of the second glucosyl residue following further acyl and glucosyl modifications. Both genes in the double mutant had nucleotide sequence changes and deletions that disrupted their transcripts and caused loss of AA7BG-GT activity in sepals. These results provide genetic confirmation that both genes are responsible for AA7BG-GT enzyme activity. |
| Databáze: | OpenAIRE |
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