Optimization of Chitosan-α-casein Nanoparticles for Improved Gene Delivery: Characterization, Stability, and Transfection Efficiency
| Autor: | Olga Borges, Edna Soares, Sofia Gil Carvalho, Ana Patrícia Marques, Sandra Jesus, João Panão Costa |
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| Rok vydání: | 2019 |
| Předmět: |
Pharmaceutical Science
Nanoparticle 02 engineering and technology Aquatic Science Gene delivery Transfection 030226 pharmacology & pharmacy Chitosan 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Drug Stability Casein Chlorocebus aethiops Drug Discovery Zeta potential Animals Particle Size Ecology Evolution Behavior and Systematics Ecology Gene Transfer Techniques technology industry and agriculture Caseins DNA Genetic Therapy General Medicine 021001 nanoscience & nanotechnology chemistry COS Cells Biophysics Nanoparticles Particle size 0210 nano-technology Agronomy and Crop Science |
| Zdroj: | AAPS PharmSciTech. 20 |
| ISSN: | 1530-9932 |
| DOI: | 10.1208/s12249-019-1342-y |
| Popis: | Among non-viral vectors, the cationic polymer chitosan has gained attention as a gene delivery system. We hypothesized that the addition of casein into the nanoparticle's structure would facilitate a proper gene transfer. The work herein presented aimed to optimize the production method of chitosan-casein nanoparticles (ChiCas NPs) and to test their ability as a gene delivery system. ChiCas NPs formulation optimization was carried out by analyzing several characteristics such as NP size, zeta potential, and chitosan and casein incorporation efficacy. The best formulation developed presented small and homogenous particle size (around 335 nm) and positive zeta potential (≈ + 38 mV), and showed to be stable for 34 weeks both, at 4°C and 20°C. The particles were further used to entrap or to adsorb DNA and form NPs-DNA complexes. In vitro transfection studies, carried out in COS-7 cells, suggested a low transfection efficiency of the different NPs:DNA ratios tested, comparatively to the positive control. Nonetheless, we could observe that the complexes with larger sizes presented better transfection results than those with smaller diameters. To conclude, ChiCas NPs have great technological potential since the preparation process is very simple, and the DNA incorporation efficacy is very high and shows to be physically very stable. The NPs:DNA ratio still needs to be optimized with the aim of achieving better transfection results and being able to anticipate a high gene expression on DNA-based vaccination studies. |
| Databáze: | OpenAIRE |
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