Physico‐Chemical Properties of Mouse Hepatitis Virus (MHV‐2) Grown on DBT Cell Culture
| Autor: | Shigeo Hino, Norio Hirano, Kosaku Fujiwara |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Ultraviolet Rays
viruses Immunology Hamster Hemagglutinins Viral Virus Replication Microbiology Virus Article Cell Line Sonication Mouse hepatitis virus Virology Centrifugation Density Gradient Centrifugation Virus quantification Infectivity Murine hepatitis virus biology Temperature Articles Hemagglutinin Hydrogen-Ion Concentration biology.organism_classification Molecular biology Cell culture |
| Zdroj: | Microbiology and Immunology |
| ISSN: | 1348-0421 0385-5600 |
| Popis: | Some properties of a strain of mouse hepatitis virus, MHV‐2, grown on DBT cells were determined using a plaque assay on the cells. Viral growth was not inhibited by the presence of actinomycin D or 5‐iodo‐2‐deoxyuridine. MHV‐2 was completely inactivated by ether, chloroform, sodium deoxycholate or beta‐propiolactone, but showed a moderate resistance to trypsin. Heating at 56 C for 30 min did not completely abolish the virus infectivity. The virus was stable after heating at 50 C for 15 min in 1m‐MgCl2 or 1m‐MgSO4 as well as at 37 C for 60 min at pH 3.0 to 9.0. Infectivity was decreased to 1/100 and 1/400 after storing at 4 C for 30 days and 37 C for 24 hr, respectively. The virus passed through a 200‐nm but not a 50‐nm Sartorius membrane filter. The buoyant density of MHV‐2 was 1.183 g/cm3 in sucrose gradient, and the fraction contained coronavirus‐like particles measuring 70 to 130 nm in diameter. Survival rate was 10% after exposure to ultraviolet at 150 ergs/mm2. Freezing and thawing or sonication at 20 kc for 3 min did not affect the virus titer. No hemagglutinin was demonstrable with red blood cells of the chicken, Japanese quail, mouse, rat, hamster, guinea pig, sheep, bovine or human. |
| Databáze: | OpenAIRE |
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