Expression of accessory molecules and cytokines in acute EAE in marmoset monkeys (Callithrix jacchus)

Autor: Mark de Boer, Eric Claassen, Bert A. 't Hart, Jon D. Laman, Marjan van Meurs, M.M. Schellekens, Hans Lassmann, Luca Massacesi, Bert Melchers
Přispěvatelé: Immunology, TNO Preventie en Gezondheid
Rok vydání: 1998
Předmět:
Male
Pathology
medicine.medical_treatment
Antigens
CD40
CD80
Antigens
CD80
CD40
Immunology and Allergy
CD86
Membrane Glycoproteins
biology
Experimental autoimmune encephalomyelitis
Brain
Marmoset
Callithrix
Antigens
CD86
Interleukin-12
Interleukin-10
medicine.anatomical_structure
Neurology
Health
B7-1 Antigen
Macrophages
Multiple sclerosis
Non-human primates
Cytokines
Female
Tumor necrosis factor alpha
medicine.medical_specialty
Encephalomyelitis
Autoimmune
Experimental
Multiple Sclerosis
T cell
Acid Phosphatase
Immunology
Interferon-gamma
Antigens
CD
biology.animal
medicine
Animals
CD40 Antigens
Brain Chemistry
Interferon Type II
Tumor Necrosis Factor-alpha
Histocompatibility Antigens Class II
Interferon-alpha
HLA-DR Antigens
Immunotherapy
biology.organism_classification
medicine.disease
Disease Models
Animal
Interleukin-2
B7-2 Antigen
Interleukin-4
Neurology (clinical)
Interleukin-1
Zdroj: Journal of Neuroimmunology, 1, 86, 30-45
Journal of Neuroimmunology, 86, 30-45. Elsevier
ISSN: 0165-5728
DOI: 10.1016/s0165-5728(98)00024-1
Popis: Accessory molecules and cytokines are involved in the immunopathogenesis of multiple sclerosis (MS) and experimental autoimmune encephalomyelitis (EAE) in rodent models, and are potential targets for immunotherapy. Evaluation of such experimental therapies requires appropriate animal models. Therefore, we analysed the expression of selected accessory molecules and cytokines in the brain of marmoset monkeys (Callithrix jacchus) with acute EAE, a newly described non-human primate model for MS. All animals experienced active disease clinically and histopathologically with strong resemblance to MS. Perivascular infiltrates of mononuclear cells showed abundant expression of CD40. CD40 was expressed on macrophages, indicating that T cell priming and macrophage effector functions may result from local CD40-CD40L interactions. CD40 ligand (CD40L) and B7-2 (CD86) were also expressed, but to a lower extent, while B7-1 (CD80) expression was limited. Both pro-inflammatory and anti-inflammatory cytokines were produced within individual lesions during active disease (IFN-alpha, IFN-gamma, TNF-alpha, IL-1alpha, IL-1beta, IL-2, IL-4, IL-10 and IL-12). This suggests that relative levels rather than sequential expression of Th1- and Th2-type cytokines determine disease activity. These findings demonstrate the value of EAE in marmoset monkeys as a model to assess the role of accessory molecules and cytokines in multiple sclerosis, and to evaluate targeted intervention.
Databáze: OpenAIRE
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