High-speed DNA sequencing in ultrathin slab gels
| Autor: | James M Dunn, Thomas D. Yager, John K. Stevens |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
DNA nanoball sequencing
DNA Complementary Materials science DNA polymerase Biomedical Engineering Gene Expression Bioengineering Nanotechnology DNA-Directed DNA Polymerase Polymerase Chain Reaction DNA sequencing chemistry.chemical_compound Genome biology DNA Sequence Analysis DNA Ion semiconductor sequencing Molecular biology Electrophoresis chemistry Evaluation Studies as Topic biology.protein Slab Electrophoresis Polyacrylamide Gel Algorithms Biotechnology Single molecule real time sequencing |
| Zdroj: | Current Opinion in Biotechnology. 8:107-113 |
| ISSN: | 0958-1669 |
| Popis: | There have been many recent theoretical and technical advances in the sequencing of DNA in ultrathin slab gels. These include recent experimental progress in four areas: DNA polymerases; DNA template preparation; the separation and detection of DNA bands; and base-calling algorithms. The Zimm—Lumpkin theory of electrophoresis provides a new and improved framework for understanding the operation of sequencing gels. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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