A NAC Domain Protein Interacts with Tomato leaf curl virus Replication Accessory Protein and Enhances Viral Replication
| Autor: | Satish C. Dogra, M. Saif Rasheed, Luke A. Selth, M. Ali Rezaian, John W. Randles, Helen Healy |
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| Rok vydání: | 2004 |
| Předmět: |
Gene Expression Regulation
Viral Transcriptional Activation Molecular Sequence Data Protein domain Saccharomyces cerevisiae Plant Science Virus Replication Plant Viruses Evolution Molecular Viral Proteins Solanum lycopersicum Gene Expression Regulation Plant Transcription (biology) medicine Amino Acid Sequence Enhancer Transcription factor Research Articles Phylogeny Plant Diseases Plant Proteins Cell Nucleus Reporter gene Base Sequence biology fungi food and beverages Cell Biology biology.organism_classification Molecular biology Protein Structure Tertiary Up-Regulation Cell nucleus medicine.anatomical_structure Viral replication DNA Viral Transcription Factors |
| Zdroj: | The Plant Cell. 17:311-325 |
| ISSN: | 1532-298X 1040-4651 |
| DOI: | 10.1105/tpc.104.027235 |
| Popis: | Geminivirus replication enhancer (REn) proteins dramatically increase the accumulation of viral DNA species by an unknown mechanism. In this study, we present evidence implicating SlNAC1, a new member of the NAC domain protein family from tomato (Solanum lycopersicum), in Tomato leaf curl virus (TLCV) REn function. We isolated SlNAC1 using yeast (Saccharomyces cerevisiae) two-hybrid technology and TLCV REn as bait, and confirmed the interaction between these proteins in vitro. TLCV induces SlNAC1 expression specifically in infected cells, and this upregulation requires REn. In a transient TLCV replication system, overexpression of SlNAC1 resulted in a substantial increase in viral DNA accumulation. SlNAC1 colocalized with REn to the nucleus and activated transcription of a reporter gene in yeast, suggesting that in healthy cells it functions as a transcription factor. Together, these results imply that SlNAC1 plays an important role in the process by which REn enhances TLCV replication. |
| Databáze: | OpenAIRE |
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