Cross-coupling of the NF-kappa B p65 and Fos/Jun transcription factors produces potentiated biological function
| Autor: | Warner C. Greene, Peter Angel, Bernd Stein, Albert S. Baldwin, Dean W. Ballard, Peter A. Herrlich |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Cytoplasm
Macromolecular Substances Proto-Oncogene Proteins c-jun Blotting Western Molecular Sequence Data Transcription factor complex Biology Proto-Oncogene Mas General Biochemistry Genetics and Molecular Biology Rel homology domain Mice Transcription (biology) Proto-Oncogene Proteins Animals Humans Amino Acid Sequence Promoter Regions Genetic Molecular Biology Transcription factor HIV Long Terminal Repeat General Immunology and Microbiology General Neuroscience NF-kappa B 3T3 Cells Molecular biology Long terminal repeat Proto-Oncogene Proteins c-rel B vitamins Electrophoresis Polyacrylamide Gel Proto-Oncogene Proteins c-fos HeLa Cells Research Article |
| Zdroj: | The EMBO journal. 12(10) |
| ISSN: | 0261-4189 |
| Popis: | NF-kappa B and AP-1 represent distinct mammalian transcription factors that target unique DNA enhancer elements. The heterodimeric NF-kappa B complex is typically composed of two DNA binding subunits, NF-kappa B p50 and NF-kappa B p65, which share structural homology with the c-rel proto-oncogene product. Similarly, the AP-1 transcription factor complex is comprised of dimers of the c-fos and c-jun proto-oncogene products or of closely related proteins. We now demonstrate that the bZIP regions of c-Fos and c-Jun are capable of physically interacting with NF-kappa B p65 through the Rel homology domain. This complex of NF-kappa B p65 and Jun or Fos exhibits enhanced DNA binding and biological function via both the kappa B and AP-1 response elements including synergistic activation of the 5' long terminal repeat of the human immunodeficiency virus type 1. These findings support a combinatorial mechanism of gene regulation involving the unexpected cross-coupling of two different classes of transcription factors to form novel protein complexes exhibiting potentiated biological activity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|