Candida albicans PROTEIN PROFILE CHANGES IN RESPONSE TO THE BUTANOLIC EXTRACT OF Sapindus saponariaL
| Autor: | Izabel Cristina Piloto Ferreira, Isis Regina Grenier Capoci, Adriana Fiorini, Kátia Cristina Sibin Melo, Luciana Dias Ghiraldi, Eliane Martins da Silva Bettega, Cristiane Suemi Shinobu-Mesquita, Patrícia de Souza Bonfim-Mendonça, Terezinha Inez Estivalet Svidzinski, Janine Silva Ribeiro Godoy, Paula Aline Zanetti Campanerut, Fábio Rogério Rosado, Caroline Kukolj |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Sapindus saponaria lcsh:Arctic medicine. Tropical medicine Antifungal Agents lcsh:RC955-962 030106 microbiology RC955-962 Microbial Sensitivity Tests Sapindus Microbiology Fungal Proteins 03 medical and health sciences Minimum inhibitory concentration Arctic medicine. Tropical medicine Candida albicans Two-dimensional gel electrophoresis Saponaria Electrophoresis Gel Two-Dimensional Fluconazole Fungal protein biology Mass spectrometry Plant Extracts Saponins biology.organism_classification Antimicrobial Corpus albicans Biochemistry Microscopy Electron Scanning Original Article |
| Zdroj: | Revista do Instituto de Medicina Tropical de São Paulo Revista do Instituto de Medicina Tropical de São Paulo; Vol. 58 (2016); e25 Revista do Instituto de Medicina Tropical de São Paulo; v. 58 (2016); e25 Instituto de Medicina Tropical (IMT) instacron:IMT Revista do Instituto de Medicina Tropical de São Paulo, Vol 58 (2016) Revista do Instituto de Medicina Tropical de São Paulo, Vol 58, Iss 0 (2016) Revista do Instituto de Medicina Tropical de São Paulo, Volume: 58, Article number: 25, Published: 08 APR 2016 |
| ISSN: | 1678-9946 0036-4665 |
| Popis: | Candida albicans is an opportunistic human pathogen that is capable of causing superficial and systemic infections in immunocompromised patients. Extracts of Sapindus saponaria have been used as antimicrobial agents against various organisms. In the present study, we used a combination of two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) to identify the changes in protein abundance of C. albicans after exposure to the minimal inhibitory concentration (MIC) and sub-minimal inhibitory concentration (sub-MIC) of the butanolic extract (BUTE) of S. saponaria and also to fluconazole. A total of six different proteins with greater than 1.5 fold induction or repression relative to the untreated control cells were identified among the three treatments. In general, proteins/enzymes involved with the glycolysis (GPM1, ENO1, FBA1), amino acid metabolism (ILV5, PDC11) and protein synthesis (ASC1) pathways were detected. In conclusion, our findings reveal antifungal-induced changes in protein abundance of C. albicans. By using the previously identified components of the BUTE of S. saponaria (e.g., saponins and sesquiterpene oligoglycosides), it will be possible to compare the behavior of compounds with unknown mechanisms of action, and this knowledge will help to focus the subsequent biochemical work aimed at defining the effects of these compounds. |
| Databáze: | OpenAIRE |
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