In Vitro Testing of the Insecticide Reldan 22 on Swine Oocyte Maturation
| Autor: | Vasile Miclea, Dragos Cosma, Ileana Miclea, Anamaria Jeni Pernes, Marius Zahan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
Fluorescein diacetate lcsh:Biotechnology General Medicine Biology Oocyte 010603 evolutionary biology 01 natural sciences In vitro Toxicology Andrology chlorpyrifos maturation oocyte pig toxicity 010602 entomology chemistry.chemical_compound medicine.anatomical_structure chemistry Chlorpyrifos lcsh:TP248.13-248.65 Toxicity Biological fluids medicine Propidium iodide lcsh:Animal culture lcsh:SF1-1100 |
| Zdroj: | Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies, Vol 73, Iss 2, Pp 144-149 (2016) |
| ISSN: | 1843-5262 |
| Popis: | Chlorpyrifos (Reldan 22) is an widely used insecticide for the control of insect pests in agricultureand in residential areas. It is classified as moderately toxic by the United States Environmental Protection Agency and has been quantified in human biological fluids. Given that the use of porcine and bovine models for testing chemicals has increased recently we designed an experiment to test the toxicity of several Chlorpyrifos concentrations and investigate its effects on maturation of swine oocytes. Swine oocytes from ovaries harvested in a commercial slaughterhouse were cultured for 44-45h in M199 supplemented with the following Reldan 22 concentrations: 0.1, 0.5, 1 or 2 µg/ml. Cumulus oophorous expansion was assessed and oocytes were denuded and stained with 1 µg/ml fluorescein diacetate to estimate viability. Afterwards, oocytes were fixed in a 60% methanol/DPBS solution and stained with 50 µg/ml propidium iodide to observe the DNA stage. Differences were analysed by the analysis of variance and interpreted using the Tuckey test. Our research shows that the insecticide Reldan 22® stimulated cumulus expansion to an extent but reduced oocyte viability which was accompanied by an increase in the number of immature oocytes and a decrease in the percentages of gametes that resumed meiosis. This leads us conclude that its presence in the oocyte environment is toxic for development at concentrations 0.5, 1 and 2 µg/ml. |
| Databáze: | OpenAIRE |
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