Multifunctionality of βCD/Ofloxacin and HPβCD/Ofloxacin Complexes: Improvement of the Antimicrobial Activity and Apoptosis Induction on Lung Adenocarcinoma A549 Cells
| Autor: | Alessandra P. Carli, Cleonice Aparecida Souza, Paloma E. Carvalho, Sandra B.R. Castro, Oswaldo Cardoso, Gabriella Freitas Ferreira, Bolivar R. Amaro, Ângelo M. L. Denadai, Caio César de Souza Alves, Fabiana S. Machado, Jeferson G. Da Silva |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
MTT
Thermogravimetric analysis Chemistry complexation flow cytometry toxicity Biological activity Isothermal titration calorimetry General Chemistry Antimicrobial Minimum inhibitory concentration medicine Ofloxacin MIC Fourier transform infrared spectroscopy Cytotoxicity Nuclear chemistry medicine.drug |
| Zdroj: | Journal of the Brazilian Chemical Society, Volume: 31, Issue: 12, Pages: 2628-2637, Published: 14 DEC 2020 Journal of the Brazilian Chemical Society v.31 n.12 2020 Journal of the Brazilian Chemical Society Sociedade Brasileira de Química (SBQ) instacron:SBQ |
| Popis: | The ofloxacin (OFLOX) is a second-generation synthetic antibiotic that can be classified as a multifunctional drug, but is a poorly soluble drug, which influences its efficiency. The inclusion complexes of OFLOX with β-cyclodextrin (βCD) or hydroxypropyl-β-cyclodextrin (HPβCD) can improve the chemical characteristics of the drug; however, studies showing the biological activity of these inclusion complexes are still scarce. The present work aimed to investigate the multifunctionality of the OFLOX and their inclusion complexes. Thus, the 1:1 βCD/OFLOX or HPβCD/OFLOX were prepared and analyzed by Fourier transform infrared spectroscopy (FTIR), thermogravimetric and differential thermal analysis (TGA and DTA), 1H nuclear magnetic resonance (NMR) and isothermal titration calorimetry (ITC). The antitumor and antibacterial effects were assessed. The results confirm the formation of the inclusion complexes, which had lower minimum inhibitory concentration (MIC) values, higher cytotoxicity and promoted the apoptosis. The present study showed, for the first time, the promising effects of the inclusion complexes as antitumor, improving the biological activities of the uncomplexed ofloxacin. |
| Databáze: | OpenAIRE |
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