Parmelia sulcata Taylor and Usnea filipendula Stirt induce apoptosis-like cell death and DNA damage in cancer cells

Autor: S. Bozdemir, Şule Öztürk, Ferda Ari, Serap Celikler, Seyhan Oran, Engin Ulukaya, Nazlihan Aztopal
Přispěvatelé: Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü., Uludağ Üniversitesi/Tıp Fakültesi/Biyokimya Anabilim Dalı., Arı, Ferda, Aztopal, Nazlıhan, Oran, Seyhan, Bozdemir, Şefika, Çelikler, Serap, Öztürk, Şule, Ulukaya, Engin, AAG-7012-2021, AAD-7322-2019, AAH-5039-2021, K-5792-2018, AAH-2767-2021, L-6687-2018
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Unclassified drug
Cell
Enzyme activation
Lichen
Apoptosis
Cancer cell
Growth
Antiproliferative activity
Cancer cell line
Liver neoplasms
Lung neoplasms
Neoplasms
Parmelia sulcata
Metabolites
Cancer inhibition
Enzyme activity
Cell proliferation
MTT assay
Caspase 3
Medicinal plant
General Medicine
Glioma
Parmelia sulcata extract
Plant extract
medicine.anatomical_structure
Antineoplastic agent
Protein cleavage
Antioxidant
Animal cell
Human
Cell death
Programmed cell death
Cell biology
DNA damage
Usnea filipendula
Biology
Nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase
Article
Fluorescence
Ascomycota
Botany
Dose response
medicine
Chemotherapy
Animals
Humans
ATPase activity assay
Comet assay
Drug effects
Keratin-18
Cytokeratin 18
Animal
Poly(ADP-ribose) polymerases
Methanol
Cell viability assay
Original Articles
Tumor cell line
biology.organism_classification
Usnea filipendula extract
Nonhuman
Molecular biology
Ascomycetes
Rats
Brain neoplasms
Cell line
tumor
Usnea
Metabolism
Human cell
Rat
Usnic Acid
Extract
Lichen (Disease)
Cell nucleus
Genotoxicity
Controlled study
Popis: Objectives: Successful cancer treatments still require more compounds to be isolated from natural sources. Thus, we have investigated anti-proliferative/apoptotic effects of methanolic extracts of lichen species Parmelia sulcata Taylor and Usnea filipendula Stirt on human lung cancer (A549, PC3), liver cancer (Hep3B) and rat glioma (C6) cells. Materials and methods: Anti-proliferative effects were monitored by MTT and adenosine triphosphate viability assays, while genotoxic activity was studied using the comet assay. Additionally, cell death mode and apoptosis assays (fluorescence staining, caspase-cleaved cytokeratin 18, caspase-3 activity and PARP cleavage) were performed. Results: Extracts produced anti-population growth effects in a dose-dependent manner (1.56–100 lg/ ml) by inducing apoptosis-like cell death. This resulted in the lines having the presence of pyknotic cell nuclei. In addition, significant increase in genetic damage in the cell lines was seen, indicating that DNA damage may have been responsible for apoptotic cell death. Conclusion: In this study, methanolic extracts of Parmelia sulcata and Usnea filipendula induced apoptosis-like cell death by causing DNA damage, to cancer cells.
Databáze: OpenAIRE
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