Platelet aggregation as a quantitative immunologic technique
| Autor: | F. A. Dolbeare |
|---|---|
| Rok vydání: | 1973 |
| Předmět: |
Blood Platelets
Male Erythrocytes Platelet Aggregation Immunology Antigen-Antibody Complex Virus Antibodies Viral Proteins Immune system Immunologic Technique Platelet Adhesiveness Antigen Animals Chelation Platelet Citrates Bovine serum albumin Antigens Edetic Acid Sheep biology Chemistry Arthritis Immune Sera Complement Fixation Tests Serum Albumin Bovine Hemagglutination Tests Mycobacterium tuberculosis Complement fixation test Orthomyxoviridae Rats Biochemistry biology.protein Cattle Rabbits gamma-Globulins |
| Zdroj: | Immunological communications. 2(1) |
| ISSN: | 0090-0877 |
| Popis: | A rapid quantitative immunologic method based on the sensitivity of platelets to immune complexes is described. Platelet-rich-plasma (PRP) from sensitized animals exposed to various concentrations of antigen (or antigen t anti-serum) is sedimented. The number of aggregated platelets is obtained from the difference between the number of initial platelets and the non-sedimenting platelets. The total number of sedimenting platelets thus obtained is a function of the antigen or antibody levels. The platelet aggregation technique is effective with antigens such as bovine serum albumin (BSA), bovine gamma globulin (BGG), human influenza virus (HIV), and tuberculin purified protein derivative (PPD). The effects of reaction time, temperature, chelation, and platelet dilution were studied. The method is as sensitive as the passive hemagglutination and complement fixation methods. |
| Databáze: | OpenAIRE |
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