Inclusion of Cross-Linked Elastin in Gelatin/PEG Hydrogels Favourably Influences Fibroblast Phenotype
| Autor: | Havazelet Bianco Peled, Ye Cao, Yee Shan Wong, Scott Alexander Irvine, Subramanian Venkatraman, Bae Hoon Lee |
|---|---|
| Přispěvatelé: | School of Materials Science and Engineering |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
food.ingredient
Polymers and Plastics 0206 medical engineering elastin macromolecular substances 02 engineering and technology Polyethylene Glycol Hydrogel Gelatin cell encapsulation Article lcsh:QD241-441 Extracellular matrix gelatin food lcsh:Organic chemistry medicine Fibroblast Cell encapsulation polyethylene glycol hydrogel dermal substitu Myofibroblast Materials [Engineering] integumentary system biology Chemistry technology industry and agriculture General Chemistry 021001 nanoscience & nanotechnology 020601 biomedical engineering myofibroblast medicine.anatomical_structure Self-healing hydrogels biology.protein Biophysics 0210 nano-technology Wound healing Elastin |
| Zdroj: | Polymers Volume 12 Issue 3 Polymers, Vol 12, Iss 3, p 670 (2020) |
| ISSN: | 2073-4360 |
| DOI: | 10.3390/polym12030670 |
| Popis: | The capacity of a biomaterial to innately modulate cell behavior while meeting the mechanical property requirements of the implant is a much sought-after goal within bioengineering. Here we covalently incorporate soluble elastin into a gelatin&ndash poly (ethylene glycol) (PEG) hydrogel for three-dimensional (3D) cell encapsulation to achieve these properties. The inclusion of elastin into a previously optimized gelatin&ndash PEG hydrogel was then evaluated for effects on entrapped fibroblasts, with the aim to assess the hydrogel as an extracellular matrix (ECM)-mimicking 3D microenvironment for cellular guidance. Soluble elastin was incorporated both physically and covalently into novel gelatin/elastin hybrid PEG hydrogels with the aim to harness the cellular interactivity and mechanical tunability of both elastin and gelatin. This design allowed us to assess the benefits of elastin-containing hydrogels in guiding fibroblast activity for evaluation as a potential dermal replacement. It was found that a gelatin&ndash PEG hydrogel with covalently conjugated elastin, supported neonatal fibroblast viability, promoted their proliferation from 7.3% to 13.5% and guided their behavior. The expression of collagen alpha-1(COL1A1) and elastin in gelatin/elastin hybrid gels increased 16-fold and 6-fold compared to control sample at day 9, respectively. Moreover, cells can be loaded into the hydrogel precursor solution, deposited, and the matrix cross-linked without affecting the incorporated cells adversely, thus enabling a potential injectable system for dermal wound healing. |
| Databáze: | OpenAIRE |
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