MicroRNA and mRNA Dysregulation in Astrocytes Infected with Zika Virus
| Autor: | Gary P. Kobinger, Shannon L. Hiebert, Stephanie A. Booth, Sarah J. Medina, Timothy F. Booth, Vanessa Zubach, Lee W. Goneau, Robert A. Kozak, Jessy A Slota, Anna Majer, Daniel R. Beniac, David Safronetz, Mia J. Biondi, Alberto Severini, Babu V Sajesh |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Microcephaly lcsh:QR1-502 Gene Expression Biology Virus Replication lcsh:Microbiology Article Virus Cell Line Zika virus 03 medical and health sciences 0302 clinical medicine flavivirus Pregnancy Virology microRNA medicine Animals Humans host response Flavivirus Infections RNA Messenger pathogenesis Zika Virus Microarray Analysis biology.organism_classification medicine.disease Neural stem cell Up-Regulation 3. Good health MicroRNAs Flavivirus 030104 developmental biology Infectious Diseases Astrocytes Immunology Female Stem cell human astrocytes 030217 neurology & neurosurgery |
| Zdroj: | Viruses; Volume 9; Issue 10; Pages: 297 Viruses Viruses, Vol 9, Iss 10, p 297 (2017) |
| ISSN: | 1999-4915 |
| DOI: | 10.3390/v9100297 |
| Popis: | The Zika virus (ZIKV) epidemic is an ongoing public health concern. ZIKV is a flavivirus reported to be associated with microcephaly, and recent work in animal models demonstrates the ability of the virus to cross the placenta and affect fetal brain development. Recent findings suggest that the virus preferentially infects neural stem cells and thereby deregulates gene expression, cell cycle progression, and increases cell death. However, neuronal stem cells are not the only brain cells that are susceptible to ZIKV and infection of other brain cells may contribute to disease progression. Herein, we characterized ZIKV replication in astrocytes, and profiled temporal changes in host microRNAs (miRNAs) and transcriptomes during infection. We observed the deregulation of numerous processes known to be involved in flavivirus infection, including genes involved in the unfolded protein response pathway. Moreover, a number of miRNAs were upregulated, including miR-30e-3p, miR-30e-5p, and, miR-17-5p, which have been associated with other flavivirus infections. This study highlights potential miRNAs that may be of importance in ZIKV pathogenesis. |
| Databáze: | OpenAIRE |
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