Fluorescence automatic cell sorter and immunohistochemical investigation of CD68-positive cells in meningioma
| Autor: | Atsuko Takei, Takayasu Miyo, Nobuhiro Nakagawa, Goro Nagashima, Hiromu Hokaku, T. Suzuki, Jun-ichirou Asai, Tsukasa Fujimoto, Ryuta Suzuki |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Adenoma
Pathology medicine.medical_specialty Brain tumor Antigens Differentiation Myelomonocytic Peripheral blood mononuclear cell Monocytes Flow cytometry Meningioma Antigens CD Meningeal Neoplasms medicine Humans Pituitary Neoplasms neoplasms Microglia medicine.diagnostic_test Brain Neoplasms CD68 business.industry Macrophages Monocyte Glioma General Medicine Flow Cytometry medicine.disease Immunohistochemistry nervous system diseases medicine.anatomical_structure Surgery Neurology (clinical) business |
| Zdroj: | Clinical Neurology and Neurosurgery. 101:229-234 |
| ISSN: | 0303-8467 |
| Popis: | Infiltration of brain neoplasms by mononuclear cells including monocytes/macrophages has attracted little attention since they have marked morphological heterogeneity. Twenty-seven meningiomas were studied by anti-CD68 antibody-gated flow cytometry and by immunohistochemical analysis using the anti-CD68 antibodies. Flow cytometric analysis divided cells contained within tumor tissues into CD68-positive and -negative cells. In addition, eight gliomas, eight metastatic brain tumor, and 12 pituitary adenomas were investigated in the same way to compare meningiomas. The mean contents of CD68-positive cells were 24.0 +/- 3.7% in meningiomas, 4.4 +/- 1.4% in gliomas, 9.5 +/- 3.9% in metastatic brain tumors, and 4.5 +/- 1.8% in pituitary adenomas. Immunohistochemically, CD68-positive cells showed significant heterogeneity and were detected as round, rod-shaped, ameboid and ramified cells in meningiomas. Although the infiltrated mononuclear cells in gliomas have been investigated to some degree and showed that they express cytokines and/or growth factors, these infiltrated cells in meningioma have barely been studied. The CD68-positive cells detected in this study are likely to be monocytes, macrophages and microglias, and are presumed to be in various functional stages and to play important roles in growth regulation in meningioma. |
| Databáze: | OpenAIRE |
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