Extracellular adenosine 5′-triphosphate-evoked glutamate release in cultured hippocampal neurons
| Autor: | Kazuhide Inoue, Kannosuke Fujimori, Ken Nakazawa, Tomokazu Watano, Akira Takanaka |
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| Rok vydání: | 1992 |
| Předmět: |
Agonist
medicine.drug_class Glutamic Acid Tetrodotoxin Biology Hippocampus Membrane Potentials chemistry.chemical_compound Adenosine Triphosphate Fetus Glutamates Quinoxalines Extracellular medicine Animals Quisqualic acid Cells Cultured 6-Cyano-7-nitroquinoxaline-2 3-dione Neurons Kainic Acid General Neuroscience Purinergic receptor Glutamate receptor Quisqualic Acid Rats Inbred Strains Depolarization Rats Electrophysiology chemistry CNQX Biophysics Neuroscience |
| Zdroj: | Neuroscience Letters. 134:215-218 |
| ISSN: | 0304-3940 |
| DOI: | 10.1016/0304-3940(92)90520-h |
| Popis: | Characteristics of extracellular ATP-evoked electrical responses in rat hippocampal neurons were investigated. Extracellular ATP (100 microM) induced a rapid depolarization followed by repetitive firings of spikes in these cells under whole-cell current-clamp. In whole-cell voltage-clamp experiments, ATP activated 2 types of inward currents that were inhibited by P2-purinoceptor blocker suramin (300 microM). One is a small (about -20 pA) sustained current which is insensitive to tetrodotoxin (TTX), and the other is a large (-100 to -300 pA) transient current which abolished in the presence of 3 microM TTX. The ATP-induced transient current was blocked by 6-cyano-7-nitro-quinoxaline-2,3-dione (CNQX; 30 microM), a non-N-methyl-D-aspartate (non-NMDA) receptor antagonist. ATP failed to induce the transient current in the cell which showed the desensitization to quisqualic acid (QA; 10 microM), a non-NMDA receptor agonist. These findings suggest that ATP directly activates small sustained currents, and indirectly induces the transient currents by evoking glutamate release. |
| Databáze: | OpenAIRE |
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