The Combination of High-Fat Diet and Oral Marijuana Promotes the Development of Fibrosis in the Mouse Corpora Cavernosa
| Autor: | Julian B. Wilson, Jocelyn Molina, Sriram Eleswarapu, Theodore C. Friedman, Michael Mangubat, Jorge N. Artaza, Andrea Abraham, Sabine Nguyen, Monica G. Ferrini |
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| Rok vydání: | 2021 |
| Předmět: |
Endocrinology
Diabetes and Metabolism 030232 urology & nephrology lcsh:Medicine Erectile tissue medicine.disease_cause Behavioral Neuroscience 0302 clinical medicine Endocrinology Basic Science Fibrosis Medicine Erectile Function Original Research 030219 obstetrics & reproductive medicine biology High-Fat Diet lcsh:Other systems of medicine Marijuana Psychiatry and Mental health medicine.anatomical_structure Public Health and Health Services Myofibroblast Tunica albuginea (penis) Urologic Diseases medicine.medical_specialty THC Urology Clinical Sciences Dermatology Paediatrics and Reproductive Medicine 03 medical and health sciences Internal medicine Nutrition Cannabis Cannabinoid Research business.industry Prevention Contraception/Reproduction lcsh:R biology.organism_classification medicine.disease lcsh:RZ201-999 Heme oxygenase Erectile dysfunction Reproductive Medicine business Oxidative stress |
| Zdroj: | Sexual medicine, vol 9, iss 2 Sexual Medicine, Vol 9, Iss 2, Pp 100312-(2021) Sexual Medicine |
| Popis: | Introduction The link between cannabis use and erectile dysfunction remains unclear. Moreover, the effect of cannabis in tandem with current Western dietary habits is an area in male sexual health that has yet to be explored. This study seeks to investigate the impact of diet and cannabis on penile health in an animal model. Aim To determine the effects of diet and oral cannabis extract on fibrosis and oxidative stress within the corpora cavernosa of mice. Methods This is a pilot animal study in which groups of 2-month old C57BL/6J male mice were fed a normal chow diet (NCD) or high-fat diet (HFD) daily and treated with or without either MJ or THC extract for 2 months. After euthanization, mouse penises were isolated and processed for immunohistochemical studies to determine: (i) smooth muscle cell to collagen content, (ii) myofibroblast proliferation, and (iii) anti-oxidative activity. Main Outcome Measures Quantitative assessment of immunohistochemical markers of fibrosis and oxidative stress within the corpora cavernosa of mice fed a high-fat diet in combination with either oral marijuana (MJ) or Δ-9-tetrahydrocannabinol extract (THC). Results The combination of HFD with MJ resulted in: (i) a decrease in the smooth/collagen ratio in the corpora cavernosa, (ii) an increase in alpha-smooth muscle actin expression in the tunica albuginea compatible with myofibroblast proliferation, and (iii) a decrease in heme oxygenase 1 expression indicating an increase in oxidative stress. Significant histological changes were not observed in the HFD + THC group. Conclusions HFD combined with oral MJ extract led to structural alterations in erectile tissue that are associated with accelerated corporal fibrosis. However, the addition of THC to the diet did not exacerbate histological changes within the corpora. Further studies are warranted to elucidate the discrepant effects between MJ and THC in order to optimize the therapeutic potential of cannabis and minimize its adverse effects on penile health. |
| Databáze: | OpenAIRE |
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