Single-cell, high-throughput analysis of cell docking to vessel wall
| Autor: | Adam Nowakowski, Miroslaw Janowski, Jaroslaw Orzel, Barbara Lukomska, Anna Andrzejewska, Piotr Walczak, Tomasz Grygorowicz, Sylwia Dabrowska |
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| Rok vydání: | 2018 |
| Předmět: |
Cell
Paracrine activity Mesenchymal Stem Cell Transplantation Mice 03 medical and health sciences 0302 clinical medicine Cell Movement Cell Adhesion medicine Animals Humans Cell Engineering Cells Cultured Messenger RNA Chemistry Mesenchymal stem cell Brain Mesenchymal Stem Cells Original Articles High throughput analysis Cell biology medicine.anatomical_structure Neurology Docking (molecular) Brain Injuries Blood Vessels Neurology (clinical) Single-Cell Analysis Cardiology and Cardiovascular Medicine 030217 neurology & neurosurgery |
| Zdroj: | J Cereb Blood Flow Metab |
| ISSN: | 1559-7016 0271-678X |
| Popis: | Therapeutic potential of mesenchymal stem cells (MSCs) has been reported consistently in animal models of stroke, with mechanism mainly through immunomodulation and paracrine activity. Intravenous injection has been a prevailing route for MSCs administration, but cell quantities needed when scaling-up from mouse to human are extremely high putting into question feasibility of that approach. Intra-arterial delivery directly routes the cells to the brain thus lowering the required dose. Cell engineering may additionally improve cell homing, further potentiating the value of intra-arterial route. Therefore, our goal was to create microfluidic platform for screening and fast selection of molecules that enhance the docking of stem cells to vessel wall. We hypothesized that our software will be capable of detecting distinct docking properties of naïve and ITGA4-engineered MSCs. Indeed, the cell flow tracker analysis revealed positive effect of cell engineering on docking frequency of MSCs (42% vs. 9%, engineered vs. control cells, p |
| Databáze: | OpenAIRE |
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