Activation of Hypoxia-Inducible Factor Signaling Modulates the RNA Protein Interactome in Caenorhabditis elegans
| Autor: | Roman-Ulrich Müller, Tim Krüger, Thomas Benzing, Katrin Bohl, Michael Ignarski, Reza Esmaillie, Bernhard Schermer, Daniyal Ahmad, Lisa Seufert, Francesca Fabretti |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Proteomics Cellular adaptation media_common.quotation_subject Mutant 02 engineering and technology Biology Interactome Article 03 medical and health sciences lcsh:Science Molecular Biology Caenorhabditis elegans media_common Multidisciplinary Molecular Interaction Integrative Aspects of Cell Biology Longevity RNA Molecular Network Biological Sciences 021001 nanoscience & nanotechnology biology.organism_classification Cell biology 030104 developmental biology Proteome lcsh:Q 0210 nano-technology |
| Zdroj: | iScience iScience, Vol 22, Iss, Pp 466-476 (2019) |
| ISSN: | 2589-0042 |
| Popis: | Summary The cellular response to hypoxia is crucial to organismal survival, and hypoxia-inducible factors (HIF) are the key mediators of this response. HIF-signaling is central to many human diseases and mediates longevity in the nematode. Despite the rapidly increasing knowledge on RNA-binding proteins (RBPs), little is known about their contribution to hypoxia-induced cellular adaptation. We used RNA interactome capture (RIC) in wild-type Caenorhabditis elegans and vhl-1 loss-of-function mutants to fill this gap. This approach identifies more than 1,300 nematode RBPs, 270 of which can be considered novel RBPs. Interestingly, loss of vhl-1 modulates the RBPome. This difference is not primarily explained by protein abundance suggesting differential RNA-binding. Taken together, our study provides a global view on the nematode RBPome and proteome as well as their modulation by HIF-signaling. The resulting RBP atlas is also provided as an interactive online data mining tool (http://shiny.cecad.uni-koeln.de:3838/celegans_rbpome). Graphical Abstract Highlights • RNA interactome capture in wild-type C. elegans and vhl-1 loss-of-function mutants • Identification of 1,354 nematode RBPs, 270 of which can be considered novel RBPs • The modulation of the RBPome by vhl-1 is primary explained by differential RNA-binding • The resulting RBP atlas is provided as an interactive online data mining tool Biological Sciences; Molecular Biology; Molecular Interaction; Molecular Network; Integrative Aspects of Cell Biology; Proteomics |
| Databáze: | OpenAIRE |
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