Antibacterial, antibiofilm and antitumor activities of grape and mulberry leaves ethanolic extracts towards bacterial clinical strains
| Autor: | Nermin S. Abdel-Hamid, Elshahat M. Ramadan, Khadiga A. Abou-Taleb, Gehan F. Galal |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Phenolic acids 030106 microbiology Soil Science Plant Science Horticulture High-performance liquid chromatography 03 medical and health sciences Minimum inhibitory concentration Grape extract Food science lcsh:Agriculture (General) Minimum bactericidal concentration biology Chemistry Biofilm HCT-16 cell line Colon cancer cell Bactericidal effect biology.organism_classification lcsh:S1-972 Morus alba 030104 developmental biology Vitis vinifera Pathogenic bacteria Animal Science and Zoology Agronomy and Crop Science Antitumor activity Bacteria Food Science |
| Zdroj: | Annals of Agricultural Sciences, Vol 62, Iss 2, Pp 151-159 (2017) |
| ISSN: | 0570-1783 |
| Popis: | The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were measured at concentrations of 0.01–2.56 mg/mL of grape and mulberry leaves ethanolic extracts. The MIC values were ranged from 0.08 to 0.16 mg/mL against Ps. aeruginosa Ps9, and 0.32 mg/mL against each of S. aureus St3, E. coli Ec3, and S. typhi Sa1. Whereas, the MBC values were ranged from 0.32 to 1.28 mg/mL of the tested extracts. The effects of the tested extracts were also studied representing the bactericidal effect of the grape extract with a ratio of 2 against all investigated isolates, except S. typhi Sa1. Whereas, the mulberry extract had a bactericidal effect towards S. aureus St3 and E. coli Ec3 with ratio of 2, and a bacteriostatic effect against Ps. aeruginosa Ps9 and S. typhi Sa1 with a ratio ≥4. The investigated bacteria found to have a strong ability to form biofilms with densities ranged from 0.67 to 0.80. Both tested extracts inhibited these biofilms with percentages ranged from 48 to 66% at sub-inhibitory concentrations (SICs) ranged from 0.04 to 0.16 mg/mL. In addition, the tested extracts have an excellent cytotoxic activity towards colon cancer cell lines (HCT-16). Five phenolic compounds detected in the tested extracts of grape and mulberry using high performance liquid chromatography (HPLC) after 9.53 min of the retention time. The phenolic compounds of both tested extracts were gallic, coumaric, ferulic, chlorogenic and caffeic with concentrations ranged from 1.28 to 6.56 µg/mL. |
| Databáze: | OpenAIRE |
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