Molecular characterization of Echinococcus species in dogs from four regions of Kenya
Autor: | David Odongo, Francis Addy, Thomas Romig, Dorothy Kagendo, Eberhard Zeyhle, Dennis Ebi, Peter Kern, Sammy M. Njenga, Cecilia Mbae, Marion Wassermann, Japhet Magambo, Erastus Mulinge |
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Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Veterinary medicine 030231 tropical medicine 03 medical and health sciences 0302 clinical medicine Dogs Sensu Echinococcosis parasitic diseases Prevalence Animals Dog Diseases Echinococcus granulosus Sensu stricto General Veterinary biology Cystic echinococcosis business.industry General Medicine 030108 mycology & parasitology biology.organism_classification Kenya Echinococcus Parasitology Livestock business Echinococcus species Mixed infection |
Zdroj: | Veterinary parasitology. 255 |
ISSN: | 1873-2550 |
Popis: | Cystic echinococcosis is endemic both in livestock and humans in many parts of Kenya. However, very little data exists on Echinococcus infections in dogs, and therefore their role in maintaining the transmission cycles and environmental contamination with eggs of Echinococcus species is unknown. The study aimed to establish the prevalence and distribution of Echinococcus granulosus sensu lato causing infection in dogs in Kenya. A total of 1621 dog faecal samples were collected from the environment in four different regions and examined microscopically for the presence of taeniid eggs. Up to 20 individual taeniid eggs per faecal sample were picked, lysed and genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and sequencing of the NADH dehydrogenase subunit 1 (nad1) gene. Eleven percent (178/1621) of faecal samples had taeniid eggs, while 4.4% (71/1621) contained Echinococcus spp. eggs. Area-wise, the faecal prevalence of Echinococcus spp. was 9.2% (48/524) in Turkana, 4.0% (20/500) in Maasai Mara, 0.7% (2/294) in Isiolo and 0.3% (1/303) in Meru. E. granulosus sensu stricto (s. s.) was the dominant Echinococcus taxon, followed by E. canadensis (G6/7) that was detected in 51 and 23 faecal samples, respectively. E. ortleppi was detected in only 5 faecal samples. We report for the first time the presence of E. felidis eggs in two dog faecal samples (from Maasai Mara region). Mixed infections of these taxa were also found in faecal samples, including: E. granulosus s. s. and E. canadensis (G6/7) (n = 7), E. granulosus s. s. and E. ortleppi (n = 1) and all three species (n = 1). The dog data presented here confirm the differences in diversity and abundance of Echinococcus spp. between regions of Kenya, correspond well with previously published data from livestock, and tentatively suggest a role of domestic dogs as a link between domestic and sylvatic cycles of Echinococcus spp. |
Databáze: | OpenAIRE |
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