Red-Emissive Guanylated Polyene-Functionalized Carbon Dots Arm Oral Epithelia against Invasive Fungal Infections
| Autor: | Xuan Li, Sarah Sze Wah Wong, Fung-Kit Tang, Ken Cham-Fai Leung, Regina Huang, Lijian Jin, Wai-Chung Li |
|---|---|
| Přispěvatelé: | The University of Hong Kong (HKU), Hong Kong Baptist University (HKBU), Mycologie moléculaire - Molecular Mycology, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Keratinocytes
MESH: Mouth Mucosa Antifungal Agents Guanosine Monophosphate 02 engineering and technology 01 natural sciences Amphotericin B MESH: Polyenes Candida albicans General Materials Science MESH: Guanosine Monophosphate MESH: Biological Availability MESH: Microbial Sensitivity Tests biology Candidiasis MESH: Quantum Dots 021001 nanoscience & nanotechnology Corpus albicans amphotericin B MESH: Candidiasis MESH: Keratinocytes MESH: Epithelial Cells Drug delivery Toxicity 0210 nano-technology Intracellular MESH: Invasive Fungal Infections medicine.drug Materials science oral epithelium Biological Availability MESH: Carbon Microbial Sensitivity Tests Polyenes MESH: Biofilms 010402 general chemistry Microbiology Quantum Dots carbon dots MESH: Amphotericin B medicine Humans [CHIM]Chemical Sciences MESH: Humans MESH: Candida albicans Biofilm Mouth Mucosa Epithelial Cells biology.organism_classification MESH: Antifungal Agents Carbon 0104 chemical sciences Bioavailability Biofilms drug delivery Invasive Fungal Infections |
| Zdroj: | ACS Applied Materials & Interfaces ACS Applied Materials & Interfaces, 2019, 11 (50), pp.46591-46603. ⟨10.1021/acsami.9b18003⟩ |
| ISSN: | 1944-8244 1944-8252 |
| DOI: | 10.1021/acsami.9b18003⟩ |
| Popis: | International audience; Oral candidiasis as a highly prevalent and recurrent infection in medically compromised individuals is mainly caused by the opportunistic fungal pathogen Candida albicans. This epithelial infection, if not controlled effectively, can progress to life-threatening systemic conditions and complications. The efficacy of current frontline antifungals is limited due to their poor bioavailability and systemic toxicity. As such, an efficient intervention is essential for controlling disease progression and recurrence. Herein, a theranostic nanoplatform (CD-Gu+-AmB) was developed to track the penetration of antifungals and perturb the invasion of C. albicans at oral epithelial tissues, via decorating the homemade red-emissive carbon dots (CD) with positively charged guanidine groups (Gu+) followed by conjugation with antifungal polyene (amphotericin B, AmB) in a reacting site-controllable manner. The generated CD-Gu+-AmB favorably gathered within the Candida cells and exhibited potent antifungal effects in both planktonic and biofilm forms. It selectively accumulated in the nuclei of human oral keratinocytes and exhibited undetectable toxicity to the host cells. Moreover, we reported for the first time the penetration and exfoliation profiles of CD in a three-dimensional organotypic model of human oral epithelial tissues, demonstrating that the extra- and intracellular accumulation of CD-Gu+-AmB effectively resisted the invasion of C. albicans by forming a "shielding" layer throughout the entire tissue. This study establishes a multifunctional CD-based theranostic nanoplatform functioning as a traceable and topically applied antifungal to arm oral epithelia, thereby shedding light on early intervention of mucosal candidiasis for oral and general health. |
| Databáze: | OpenAIRE |
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