Circulating epoxide hydrolase immunodeterminants in rats bearing hyperplastic nodules induced by 2-acetylaminofluorene☆
| Autor: | Ram B. Palakodety, Robert H. Broyles, Martin J. Griffin |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Male
Cancer Research Enzyme-Linked Immunosorbent Assay Biology Epitope Epitopes chemistry.chemical_compound Tissue culture Liver Neoplasms Experimental medicine Animals Epoxide hydrolase Epoxide Hydrolases chemistry.chemical_classification Rats Inbred Strains Nodule (medicine) 2-Acetylaminofluorene Hyperplasia medicine.disease Molecular biology Rats Enzyme Oncology chemistry medicine.symptom Precancerous Conditions Hyperplastic nodules |
| Zdroj: | Cancer Letters. 38:347-353 |
| ISSN: | 0304-3835 |
| DOI: | 10.1016/0304-3835(88)90027-4 |
| Popis: | Epoxide hydrolase immunodeterminants (EH-immunodeterminants), as measured by a highly sensitive and specific enzyme-linked immunosorbent assay (ELISA), were detected in the sera of rats bearing hyperplastic liver nodules induced by feeding 2-acetylaminofluorene (2-AAF), while they were low or absent (less than 0.05 microgram/ml) in the sera of rats fed a basal diet. The levels of EH-immunodeterminants ranged from 0.2 microgram/ml to 2 microgram/ml of serum and correlated (P less than 0.01) with the nodular status of the liver. Rats with predominantly small nodules (less than 3 mm) had circulating levels of antigen from 0.15 to 0.35 microgram/ml while those with large nodules (greater than 3 mm) had values ranging from 0.4 to 2.0 micrograms/ml serum and the serum levels for rats containing both small and large nodules were found to be in the intermediate range (0.2-0.8 microgram/ml). EH-immunodeterminants were also released into tissue culture medium from incubated hyperplastic nodule slices and the levels of the immunodeterminants released from nodules were about 5-fold higher than that released from incubated control liver. |
| Databáze: | OpenAIRE |
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