Isolation and characterization of marine psychrophilic phage-host systems from Arctic sea ice
| Autor: | Thomas Schweder, Elisabeth Helmke, Renate Hanschke, Michael Borriss |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Shewanella
Marine Biology Biology Microbiology Flavobacterium Shewanella frigidimarina Bacteriophage Siphoviridae 03 medical and health sciences Marine bacteriophage Bacteriophages Psychrophile 030304 developmental biology 0303 health sciences geography geography.geographical_feature_category 030306 microbiology Arctic Regions Alteromonadaceae General Medicine biology.organism_classification Arctic ice pack Microscopy Electron Molecular Medicine Water Microbiology |
| Zdroj: | Extremophiles : life under extreme conditions. 7(5) |
| ISSN: | 1431-0651 |
| Popis: | Phage-host systems from extreme cold environments have rarely been surveyed. This study is concerned with the isolation and characterization of three different phage-host systems from Arctic sea ice and melt pond samples collected north-west of Svalbard (Arctic). On the basis of 16S rDNA sequences, the three bacterial phage hosts exhibited the greatest similarity to the species Shewanella frigidimarina (96.0%), Flavobacterium hibernum (94.0%), and Colwellia psychrerythraea (98.4%), respectively. The host bacteria are psychrophilic with good growth at 0 degrees C, resulting in a rapid formation of visible colonies at this temperature. The phages showed an even more pronounced adaptation to cold temperatures than the bacteria, with growth maxima below 14 degrees C and good plaque formation at 0 degrees C. Transmission electron microscopy (TEM) examinations revealed that the bacteriophages belonged to the tailed, double-stranded DNA phage families Siphoviridae and Myoviridae. All three phages were host-specific. |
| Databáze: | OpenAIRE |
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