DNA extraction from amoebal isolates and genotype determination of Acanthamoeba from tap water in Latvia
| Autor: | Aivars Bērziņš, Artjoms Mališevs, Inese Gavarāne, Jūlija Trofimova, Ilze Rubeniņa, Olga Valciņa, Muza Kirjušina |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Genotype 030106 microbiology Acanthamoeba Biology 18S ribosomal RNA Microbiology 03 medical and health sciences Tap water parasitic diseases RNA Ribosomal 18S medicine Animals Granulomatous amoebic encephalitis Genotyping General Veterinary Water Pollution Water General Medicine DNA Protozoan medicine.disease biology.organism_classification Invertebrates Latvia DNA extraction 030104 developmental biology Infectious Diseases Insect Science GenBank Parasitology Databases Nucleic Acid |
| Zdroj: | Parasitology Research. 117:3299-3303 |
| ISSN: | 1432-1955 0932-0113 |
| Popis: | Acanthamoeba are free-living amoebae found in various ecological environments, and they are major microbial predators. Some Acanthamoeba species can cause keratitis, granulomatous amoebic encephalitis, and infections of the organs. This is the first study in Latvia describing Acanthamoeba detection by molecular techniques and genotyping using tap water from different sources. An 18S rRNA gene investigation of the 20 Acanthamoeba isolates suggested that 19 belonged to the hazardous T4 genotype and one sample was T12. The results indicate that tap water in Latvia may be a source of Acanthamoeba that is potentially pathogenic to humans. All obtained sequences were submitted to the GenBank database under the following accession numbers: MG386295-MG386314. |
| Databáze: | OpenAIRE |
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