CDK-dependent activation of poly(ADP-ribose) polymerase member 10 (PARP10)
| Autor: | Han Ting Chou, Sheng-Chung Lee, Han-Yi E. Chou |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
DNA damage
Poly ADP ribose polymerase Molecular Sequence Data Kinesins In Vitro Techniques Biochemistry Cell Line Small hairpin RNA Mice Cyclin-dependent kinase Transcription (biology) Proto-Oncogene Proteins Cyclin E Animals Humans Amino Acid Sequence Phosphorylation Molecular Biology Polymerase Cell Proliferation biology Base Sequence Sequence Homology Amino Acid Cell Cycle Cyclin-Dependent Kinase 2 Cell Biology DNA Molecular biology Cyclin-Dependent Kinases Recombinant Proteins Enzyme Activation Cytoplasm biology.protein Poly(ADP-ribose) Polymerases Cytokinesis HeLa Cells |
| Zdroj: | The Journal of biological chemistry. 281(22) |
| ISSN: | 0021-9258 |
| Popis: | Proteins of the poly(ADP-ribose) polymerase (PARP) family play a wide array of functions, covering virtually every aspect of DNA metabolism and function, most notably with the response to DNA damage, transcription, and the maintenance of genomic stability. Here we report the identification and characterization of a novel PARP family member, PARP10 (FLJ14464 or hypothetical protein LOC84875). Overexpression of PARP10 results in loss of cell viability, although down-expression by short hairpin RNA leads to delayed G1 progression and concomitant cell death. PARP10 exists in both cytoplasm and nucleus, but only nucleolar PARP10 acquires CDK-dependent phosphorylation through late-G1 to S phase, and from prometaphase to cytokinesis in the nucleolar organizing regions. The PARP activity of PARP10 depends on phosphorylation by CDK2-cyclin E in vitro. CDK-phosphorylated PARP10 is absent in growth-arrested cells. These results suggest that PARP10 functions in cell proliferation and may serve as a marker for proliferating cells. |
| Databáze: | OpenAIRE |
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