Molecular cloning of superoxide dismutase (Cu/Zn-SOD) from aquatic molluscs
| Autor: | F. Geret, H. Manduzio, R. Company, F. Leboulenger, M.J. Bebianno, J.M. Danger |
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| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
Conserved sequence
DNA Complementary Time Factors Molecular Sequence Data Dreissena polymorpha Sequence Homology Sequence alignment Superoxide dismutase Aquatic Science Molecular cloning Oceanography Amino acid sequence Bathymodiolus azoricus Ruditapes decussatus Complementary DNA Molecular sequence data Animals Amino Acid Sequence Peptide sequence Conserved Sequence DNA sequence analysis DNA Primers Base Sequence biology Superoxide Dismutase Ecology Enzyme induction Sequence Analysis DNA General Medicine Mussel Molluscs Bivalvia biology.organism_classification Pollution Base sequence Biochemistry Enzyme Induction Sequence homology biology.protein DNA primers Sequence Alignment cDNA Copper |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| Popis: | The potential of the first line of the active oxygen-scavenging system, partial cDNA encoding Cu/Zn superoxide dismutase (SOD) was isolated in three aquatic mollusc species: Ruditapes decussatus (marine clam), Dreissena polymorpha (continental water mussel) and Bathymodiolus azoricus (hydrothermal vent mussel). These SOD cDNA fragments were amplified by PCR with degenerate oligonucleotide primers derived from the amino acid sequence conserved in the Cu/Zn-SOD from several other organisms. A partial cDNA of CuZn-SOD was obtained for R. decussates (510 bp), D. polymorpha (510 bp) and B. azoricus (195 bp). The deduced amino acid sequence showed high similarity among the three mollusc species (57-63%) and among other species (50-65%). The residues involved in coordinating copper (His-47, 49, 64, 121) and zinc (His-64, 72, 81 and Asp-84) were well conserved among the three Cu/Zn-SOD sequences. FCT (Fundação para Ciência e a Tecnologia) of the Ministry of Science and Technology of Portugal info:eu-repo/semantics/publishedVersion |
| Databáze: | OpenAIRE |
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