Human papillomavirus: cause of epithelial lacrimal sac neoplasia?
| Autor: | Patricia Cassonnet, Steffen Heegaard, Gérard Orth, Patricia Flamant, Jan Ulrik Prause, Nicolai Christian Sjö, Christian von Buchwald, Bodil Norrild |
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| Přispěvatelé: | Eye Pathology Institute, Eye Pathology Institute-University of Copenhagen = Københavns Universitet (KU), Department of Otorhinolaryngology, Rigshospitalet [Copenhagen], Copenhagen University Hospital-Copenhagen University Hospital, Papillomavirus, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Institute of Medical Cell Biology and Genetics, Institute of Medical Cell Biology and Genetics-University of Copenhagen = Københavns Universitet (KU), University of Copenhagen = Københavns Universitet (KU)-Institute of Medical Cell Biology and Genetics, Eye Pathology Institute-University of Copenhagen = Københavns Universitet (UCPH), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institute of Medical Cell Biology and Genetics-University of Copenhagen = Københavns Universitet (UCPH) |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Male
MESH: Human papillomavirus 11 MESH: Carcinoma Pathology MESH: DNA Replication MESH: Human papillomavirus 6 MESH: Papillomavirus Infections MESH: Eye Neoplasms Polymerase Chain Reaction law.invention 0302 clinical medicine MESH: Aged 80 and over MESH: Autoradiography law In Situ Hybridization Polymerase chain reaction Aged 80 and over MESH: Aged MESH: Middle Aged Lacrimal Apparatus Diseases Human papillomavirus 11 HPV infection virus diseases MESH: Carcinoma Squamous Cell Middle Aged Lacrimal sac female genital diseases and pregnancy complications 3. Good health medicine.anatomical_structure 030220 oncology & carcinogenesis MESH: RNA Viral [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology Carcinoma Squamous Cell RNA Viral Female Adult DNA Replication medicine.medical_specialty In situ hybridization MESH: Lacrimal Apparatus Diseases Biology 03 medical and health sciences MESH: In Situ Hybridization medicine Carcinoma Humans Aged MESH: Carcinoma Transitional Cell Carcinoma Transitional Cell MESH: Humans Papilloma Eye Neoplasms Papillomavirus Infections MESH: Adult MESH: Polymerase Chain Reaction Transitional epithelium Human papillomavirus 6 medicine.disease Dacryocystitis MESH: Male MESH: DNA Viral Ophthalmology DNA Viral 030221 ophthalmology & optometry Autoradiography MESH: Papilloma MESH: Female |
| Zdroj: | Acta Ophthalmologica Scandinavica Acta Ophthalmologica Scandinavica, Wiley-Blackwell, 2007, 85 (5), pp.551-6. ⟨10.1111/j.1600-0420.2007.00893.x⟩ Acta Ophthalmologica Scandinavica, 2007, 85 (5), pp.551-6. ⟨10.1111/j.1600-0420.2007.00893.x⟩ |
| ISSN: | 1395-3907 1600-0420 |
| Popis: | Purpose: Epithelial tumours of the lacrimal sac are rare but important entities that may carry grave prognoses. In this study the prevalence and possible role of human papillomavirus (HPV) infection in epithelial tumours of the lacrimal sac were evaluated. Methods: Five papillomas and six carcinomas of the lacrimal sac were investigated for the presence of HPV using the polymerase chain reaction (PCR) technique. Fifteen specimens of dacryocystitis were included in the PCR reactions as controls. Furthermore, DNA in situ hybridization (ISH) and RNA ISH were performed. Results: Low-risk HPV types 6 or 11 were identified in all four lacrimal sac papillomas suitable for PCR analysis and in situ hybridization. Four of six lacrimal sac carcinomas harboured HPV. One carcinoma was positive for HPV 11 only, two carcinomas had concomitant infection with HPV 6 or 11 and high-risk HPV 16, and the remaining carcinoma was positive for HPV 16. All specimens of dacryocystitis were betaglobin-positive and HPV-negative. Using DNA ISH, two papillomas and a single carcinoma showed evidence for vegetative HPV 11 DNA replication, whereas no HPV 16 DNA replication was found in the five carcinomas tested. HPV 11 RNA was demonstrated in two papillomas. Conclusions: By analysing 11 epithelial lacrimal sac papillomas and carcinomas using PCR, DNA ISH and RNA ISH, we found HPV DNA in all investigated transitional epithelium tumours of the lacrimal sac. HPV RNA was present in two of eight epithelial lacrimal sac tumours positive for HPV DNA. As RNA degrades fast in paraffin-embedded tissue, only a small fraction of DNA-positive tumours can be expected to be RNA-positive. We therefore suggest that HPV infection is associated with the development of lacrimal sac papillomas and carcinomas. |
| Databáze: | OpenAIRE |
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