Nanohoop Rotaxane Design to Enhance the Selectivity of Reaction-Based Probes: A Proof-of-Principle Study
| Autor: | Claire E. Otteson, Carolyn M Levinn, Jeff M. Van Raden, Ramesh Jasti, Michael D. Pluth |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Steric effects
Rotaxane Fluorophore biology 010405 organic chemistry Organic Chemistry Active site Thread (computing) 010402 general chemistry 01 natural sciences Biochemistry Fluorescence Combinatorial chemistry Article 0104 chemical sciences chemistry.chemical_compound chemistry Proof of concept biology.protein Physical and Theoretical Chemistry Selectivity |
| Zdroj: | Org Lett |
| ISSN: | 1523-7052 |
| Popis: | Mechanical interlocking of a nanohoop fluorophore and a reactive thread couples the benefits of a reaction-based probe with a sterically congested active site for enhanced selectivity. Advantageously, the thread design uses dual function stoppers that act as both a quencher and a trigger for sensing. In progress toward expanding this approach to biologically relevant analytes, this system is used to demonstrate steric differentiation and provide a selective turn-on fluorescent response with size selectivity for HS(−) rather than larger thiolates. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|