Fasting and Glucagon Stimulate Gene Expression of Pyruvate Dehydrogenase Kinase 4 in Chickens
| Autor: | Takaoki Saneyasu, Kazuhisa Honda, Kiyotaka Kurachi, Shoko Takagi, Hiroshi Kamisoyama, Haruka Sugimoto |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty animal structures Pyruvate dehydrogenase kinase chicken PDK4 030209 endocrinology & metabolism White adipose tissue Glucagon ATGL 03 medical and health sciences 0302 clinical medicine Internal medicine medicine Lipolysis Diacylglycerol kinase Chemistry Research Note 030104 developmental biology Endocrinology glucagon Adipogenesis Adipose triglyceride lipase Animal Science and Zoology free fatty acid |
| Zdroj: | The Journal of Poultry Science |
| ISSN: | 1349-0486 1346-7395 |
| Popis: | The excessive accumulation of body fat has become a serious problem in the broiler industry. However, the molecular mechanisms underlying the regulation of lipid metabolism-related genes in broiler chickens are not fully understood. In the present study, we investigated the role of glucagon on the expression of lipid metabolism-related genes in chicken white adipose tissue (WAT). Four hours of fasting significantly increased plasma levels of free fatty acid in broiler chickens. The mRNA levels of adipose triglyceride lipase (ATGL) and pyruvate dehydrogenase kinase 4 (PDK4) in abdominal WAT significantly increased by fasting, whereas the mRNA levels of diacylglycerol O-acyl-transferase homolog 2 (DGAT2) and peroxisome proliferator-activated receptor-γ (PPARγ) significantly decreased. The results suggest that fasting stimulates lipolysis and suppresses adipogenesis and re-esterification of TG in chicken WAT. Glucagon significantly increased the mRNA levels of PDK4 in chicken primary adipocytes, whereas there were no significant changes in the mRNA levels of ATGL, DGAT2, and PPARγ. Our findings suggest that glucagon upregulates PDK4 expression and may stimulate lipolysis without affecting the expression of ATGL in chicken WAT. |
| Databáze: | OpenAIRE |
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