Biliary secretion of extracorporeal porcine livers with single and dual vessel perfusion
| Autor: | Jamie MacPhee, Frank J. Vittimberga, Mark P. Callery, Rocco Ricciardi, Angela N. Traylor, Thomas J. McLaughlin, Steven H. Quarfordt, David P. Foley, Susan E. Donohue, William C. Meyers |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
medicine.medical_specialty
Extracorporeal Circulation medicine.drug_class Swine Excretion Bile Acids and Salts chemistry.chemical_compound Hepatic Artery In vivo Internal medicine medicine Animals Phospholipids Transplantation Bile acid Cholesterol business.industry Portal Vein Extracorporeal circulation Surgery Perfusion Endocrinology medicine.anatomical_structure chemistry Liver Hepatocyte Phosphatidylcholines business |
| Zdroj: | Transplantation. 68(3) |
| ISSN: | 0041-1337 |
| Popis: | BACKGROUND Hepatic support systems that provide detoxification without biliary secretion (i.e., isolated hepatocyte systems) are sufficient to improve encephalopathy and bridge patients to transplantation. However, biliary secretion may be critical when hepatic support attempts to restore function and regeneration of the host liver. The purpose of these studies was to optimize the support liver secretory response to bile acid by either single-vessel (portal vein; PV) or dual-vessel (hepatic artery [HA] + PV) perfusions during extracorporeal porcine liver perfusion. METHODS Extracorporeal porcine liver perfusion of anesthetized pigs was developed using support porcine livers perfused through the PV (n=4) alone and through the HA + PV (n=4) via a venovenous circuit. Support livers were provoked with taurocholate (TC) to enhance bile aqueous and hydrophobic outputs. RESULTS After cold preservation and reperfusion, both PV and HA + PV livers had initial 1-hr bile aqueous outputs < 15% of in vivo flow, with cholesterol (C) and phospholipid (PC) outputs |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|