Aneuploid subpopulations in tumour-invaded lymph nodes from breast cancer patients
| Autor: | S.M. Alam, P. Whitford, W. Cushley, W.D. George, Ailsa M. Campbell |
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| Rok vydání: | 1992 |
| Předmět: |
Cancer Research
Pathology medicine.medical_specialty Antibodies Neoplasm Mammary gland Aneuploidy Breast Neoplasms Biology Breast cancer medicine Humans Lymph node Neoplasms Second Primary DNA Neoplasm medicine.disease Flow Cytometry medicine.anatomical_structure Oncology Lymphatic Metastasis biology.protein Keratins Female Lymph Ploidy Antibody NODAL |
| Zdroj: | European journal of cancer (Oxford, England : 1990). 28(2-3) |
| ISSN: | 0959-8049 |
| Popis: | Fresh, paired primary tumours and lymph node metastases from breast cancer patients were compared by DNA flow cytometry. Although 65% of primary tumours were aneuploid, the detection of aneuploid peaks in corresponding nodal metastases was rare (only 6 cases out of 25) in single-parameter DNA analysis. Detection of aneuploid subpopulations in lymph nodes was greatly improved in dual-parameter DNA analysis using an anticytokeratin (CK) antibody which allowed ploidy determination on CK+ epithelial cells alone. Examination of 12 lymph nodes for CK+ cells revealed the presence of both diploid and aneuploid tumour cells in tumour invaded nodes. In patients with multiploid primary tumours, a subpopulation of the primary aneuploid cells was dominant in the nodal metastases. This suggests that aneuploidy is an integral property of metastatic cells and that within a primary tumour a subpopulation may have a higher metastatic potential. |
| Databáze: | OpenAIRE |
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