A chromosome map of the Flavescence dorée phytoplasma
| Autor: | Sylvie Malembic-Maher, Elisabeth Boudon-Padieu, Xavier Foissac, Fiona Constable, Guillaume Arnaud, Agnès Cimerman, Patricia Carle |
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| Přispěvatelé: | Génomique, développement et pouvoir pathogène (GD2P), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA), Plante - microbe - environnement : biochimie, biologie cellulaire et écologie (PMEBBCE), Centre National de la Recherche Scientifique (CNRS)-Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD) |
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
DNA
Bacterial Phytoplasma BACTERIOLOGIE Molecular Sequence Data CARTOGRAPHIE GENETIQUE Microbiology Restriction fragment FLAVESCENCE DOREE MALADIE DES PLANTES 03 medical and health sciences Bacterial Proteins MOLLICUTE Deoxyribonucleases Type II Site-Specific 030304 developmental biology Southern blot Genetics 0303 health sciences biology 030306 microbiology Chromosome Fabaceae Genes rRNA Sequence Analysis DNA Genome project GENETIQUE Physical Chromosome Mapping biology.organism_classification Electrophoresis Gel Pulsed-Field Blotting Southern Restriction site [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Italy PHYTOPLASME DE LA FLAVESCENCE DOREE biology.protein Mollicutes CARTE CHROMOSOMIQUE France RRNA Operon |
| Zdroj: | Microbiology Microbiology, Microbiology Society, 2008, 154 (5), pp.1454-1463. ⟨10.1099/mic.0.2007/013888-0⟩ |
| ISSN: | 1350-0872 1465-2080 |
| DOI: | 10.1099/mic.0.2007/013888-0⟩ |
| Popis: | International audience; The Flavescence dorée phytoplasma (FD-P), a non-cultivable, plant-pathogenic bacterium of the class Mollicutes, is the causal agent of a quarantine disease affecting vineyards of southern Europe, mainly in southern France and northern Italy. To investigate FD-P diversity and phytoplasma genetic determinants governing the FD-P life cycle, a genome project has been initiated. A physical map of the chromosome of FD-P strain FD92, purified from infected broad beans, was constructed by performing restriction digests of the chromosome and resolving the fragments by PFGE. Single and double digestions of the chromosome with the enzymes SalI, BssHII, MluI and EagI were performed and used to map 13 restriction sites on the FD-P chromosome. The size of the chromosome was calculated to be 671 kbp. Southern blot analyses using cloned phytoplasma probes were carried out to assist in the arrangement of contiguous restriction fragments and to map eight genetic loci, including the two rRNA operons, the tuf, uvrB-degV and secY-map (FD9) genes, the FD2 marker and two orphan sequences (FDDH29 and FDSH05) isolated through subtractive suppression hybridization. |
| Databáze: | OpenAIRE |
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