Monophosphoryl Lipid A and Pam3Cys Prevent the Increase in Seizure Susceptibility and Epileptogenesis in Rats Undergoing Traumatic Brain Injury
| Autor: | Baharak Khoshkholgh-Sima, Morteza Zendedel, Vahab Babapour, Hamid Gholami Pourbadie, Soghra Hesam, Mohammad Sayyah |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Neurology Traumatic brain injury Lipoproteins Monophosphoryl Lipid A Biochemistry Epileptogenesis 03 medical and health sciences Cellular and Molecular Neuroscience Epilepsy 0302 clinical medicine Seizures Internal medicine Brain Injuries Traumatic medicine Kindling Neurologic Animals Rats Wistar Neuroinflammation Seizure threshold business.industry Kindling Brain General Medicine medicine.disease Amygdala Epilepsy Post-Traumatic 030104 developmental biology Endocrinology Lipid A business 030217 neurology & neurosurgery |
| Zdroj: | Neurochemical research. 43(10) |
| ISSN: | 1573-6903 |
| Popis: | Five percent of all epilepsy cases are attributed to traumatic brain injury (TBI), which are known as post-traumatic epilepsy (PTE). Finding preventive strategies for PTE is valuable. Remarkable feature of TBI is activation of microglia and subsequent neuroinflammation, which provokes epileptogenesis. The toll-like receptor agonists monophosphoryl lipid A (MPL) and tri-palmitoyl-S-glyceryl-cysteine (Pam3Cys) are safe, well-tolerated and effective adjuvants existing in prophylactic human vaccines. We examined the impact of early injection of MPL and Pam3Cys to rats, on the rate of kindled seizures acquisition following TBI. Rats received a single dose (1 µg/rat) of MPL or Pam3Cys through intracerebroventricular injection. 5 days later, trauma was exerted to temporo-parietal cortex of rats by controlled cortical impact device. After 24 h, traumatic rats underwent amygdala kindling. Brain level of the inflammatory cytokine tumor necrosis factor-alpha (TNF-α) was also measured in traumatic rats by immunoblotting. Compared to non-traumatic (sham-operated) rats, traumatic rats showed three times lower seizure threshold (133 ± 5 µA vs. 416.3 ± 16 µA, p |
| Databáze: | OpenAIRE |
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