An Orthotopic Resectional Mouse Model of Pancreatic Cancer
| Autor: | Therese M. Becker, Tony C. Y. Pang, Jeremy S. Wilson, David Goldstein, Minoti V. Apte, Romano C. Pirola, Srinivasa Pothula, Zhihong Xu, Alpha Raj Mekapogu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty medicine.medical_treatment General Chemical Engineering Splenectomy Urology Mice Nude General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine Pancreatectomy Pancreatic tumor Pancreatic cancer medicine Adjuvant therapy Animals Neoadjuvant therapy Mice Inbred BALB C General Immunology and Microbiology business.industry General Neuroscience Cancer medicine.disease Pancreatic Neoplasms Disease Models Animal 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Female business Pancreas Neoplasm Transplantation Spleen |
| Zdroj: | Journal of visualized experiments : JoVE. (163) |
| ISSN: | 1940-087X |
| Popis: | There is a lack of satisfactory animal models to study adjuvant and/or neoadjuvant therapy in patients being considered for surgery of pancreatic cancer (PC). To address this deficiency, we describe a mouse model involving orthotopic implantation of PC followed by distal pancreatectomy and splenectomy. The model has been demonstrated to be safe and suitably flexible for the study of various therapeutic approaches in adjuvant and neo adjuvant settings. In this model, a pancreatic tumor is first generated by implanting a mixture of human pancreatic cancer cells (luciferase-tagged AsPC-1) and human cancer associated pancreatic stellate cells into the distal pancreas of Balb/c athymic nude mice. After three weeks, the cancer is resected by re-laparotomy, distal pancreatectomy and splenectomy. In this model, bioluminescence imaging can be used to follow the progress of cancer development and effects of resection/treatments. Following resection, adjuvant therapy can be given. Alternatively, neoadjuvant treatment can be given prior to resection. Representative data from 45 mice are presented. All mice underwent successful distal pancreatectomy/splenectomy with no issues of hemostasis. A macroscopic proximal pancreatic margin greater than 5 mm was achieved in 43 (96%) mice. The technical success rate of pancreatic resection was 100%, with 0% early mortality and morbidity. None of the animals died during the week after resection. In summary, we describe a robust and reproducible technique for a surgical resection model of pancreatic cancer in mice which mimics the clinical scenario. The model may be useful for the testing of both adjuvant and neoadjuvant treatments. |
| Databáze: | OpenAIRE |
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