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Introduction With producing the severe neurological symptoms and movement disorders, ossification of the posterior longitudinal ligament(OPLL) have attracted attention in mounting numbers. Increasing evidences show that circRNAs act a vital regulatory role in the biological process of human disease development. However, the potential mechanism of circRNAs in OPLL was not fully known.MethodsIn this study, 3 OPLL patients and 3 controls were selected for RNA-seq analysis and the expression profiles of circRNAs were established. TargetScan and miRanda's miRNA target prediction software were used to predict the circRNA-microRNA interaction. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis was used to identify the biological process and key pathways. we used STRING database to analyze and construct a Protein-Protein Interaction (PPI) network. Several circRNAs were randomly selected for quantitative real-time PCR (qRT-PCR) validation. ResultsMicroarray data profiling indicated that 489 circRNAs (234 up and 255 down) was highly differentially expressed with setting screening criteria (FC ≥ 2.0 and p value ≤ 0.05). GO enrichment and KEGG pathways analysis revealed some processes and pathways might influence the progress of OPLL such as osteoclast differentiation, vasopressin-regulated water reabsorption, Wnt, MAPK, VEGF signaling pathway. We have obtained some significant genes such as AKT3, ACVR1, RBPJ, NFATC1, PTK2, SLC8A1. The osteoclast activity regulated by AKTs may compensate for the promotion of bone ectopic hyperplasia on OPLL.Conclusions It is the first study for portraying circRNAs expression profiles of OPLL. This provides new ideas for the prevention and alleviation of OPLL . The specific functions and mechanisms of circRNAs in OPLL should be further verified to provide more clinical treatment options for OPLL patients. |
